Rodd H D, Boissonade F M
Department of Oral Health and Development, School of Clinical Dentistry, University of Sheffield, Claremont Crescent, Sheffield S10 2TA, UK.
Int J Paediatr Dent. 2006 Jan;16(1):2-9. doi: 10.1111/j.1365-263X.2006.00682.x.
The aim of this study was to determine whether there are any differences in the number and distribution of immune cells within human primary and permanent tooth pulp, both in health and disease.
The research took the form of a quantitative immunocytochemical study. One hundred and twenty-four mandibular first permanent molars and second primary molars were obtained from children requiring dental extractions under general anaesthesia. Following exodontia, 10-microm-thick frozen pulp sections were processed for indirect immunofluorescence. Triple-labelling regimes were employed using combinations of the following: (1) protein gene product 9.5, a general neuronal marker; (2) leucocyte common antigen (LCA); and (3) Ulex europaeus I lectin, a marker of vascular endothelium. Image analysis was then used to determine the percentage area of immunostaining for LCA.
Leucocytes were significantly more abundant in the pulp horn and mid-coronal region of intact and carious primary teeth, as compared to permanent teeth (P < 0.05, anova). Both dentitions demonstrated the presence of well-localized inflammatory cell infiltrates and marked aborization of pulpal nerves in areas of dense leucocyte accumulation.
Primary and permanent tooth pulps appear to have a similar potential to mount inflammatory responses to gross caries The management of the compromised primary tooth pulp needs to be reappraised in the light of these findings.
本研究旨在确定在健康和疾病状态下，人类乳牙和恒牙牙髓内免疫细胞的数量和分布是否存在差异。
本研究采用定量免疫细胞化学研究形式。从需要在全身麻醉下拔牙的儿童中获取124颗下颌第一恒磨牙和第二乳磨牙。拔牙后，将10微米厚的牙髓冰冻切片进行间接免疫荧光处理。采用三联标记方案，使用以下组合：（1）蛋白质基因产物9.5，一种通用神经元标记物；（2）白细胞共同抗原（LCA）；（3）欧洲荆豆I凝集素，一种血管内皮标记物。然后使用图像分析来确定LCA免疫染色的百分比面积。
与恒牙相比，完整和龋坏乳牙的牙髓角和冠中区域的白细胞明显更多（P < 0.05，方差分析）。两种牙列均显示存在定位良好的炎性细胞浸润，并且在白细胞密集聚集区域牙髓神经有明显分支。
乳牙和恒牙牙髓对严重龋齿似乎具有相似的引发炎症反应的潜力。鉴于这些发现，需要重新评估受损乳牙牙髓的处理方法。
