柴山羊胎儿、新生儿及成年睾丸中类固醇生成酶的免疫定位

Immunolocalization of steroidogenic enzymes in the fetal, neonatal and adult testis of the Shiba goat.

作者信息

Weng Qiang, Medan Mohamed S, Ren LongQuan, Watanabe Gen, Tsubota Toshio, Taya Kazuyoshi

机构信息

Faculty of Biological Science and Technology, Beijing Forestry University, Beijing, China.

出版信息

Exp Anim. 2005 Oct;54(5):451-4. doi: 10.1538/expanim.54.451.

DOI:10.1538/expanim.54.451

PMID:16365523

Abstract

The localizations of steroidogenic enzymes (P450scc, 3 beta HSD, P450c17 and P450arom) in testes of Shiba goats were investigated by immunohistochemistry. P450scc, 3 beta HSD, P450c17 and P450arom were detected in all Leydig cells of adults. P450scc and P450c17 were observed in most Leydig cells in the fetus (90 days) and neonate (15 days). 3 beta HSD and P450arom were found in some Leydig cells of the fetus with weak immunostaining but the numbers of immunopositive Leydig cells and intense immunostaining were increased in Leydig cells of the neonate. These results suggest that Shiba goat testes have the ability to synthesize progestin, androgen and estrogen in the fetus, neonate and adult, and synthesis of these steroid hormones showed an age-related rise.

摘要

采用免疫组织化学方法研究了柴山羊睾丸中类固醇生成酶（P450scc、3βHSD、P450c17和P450arom）的定位。在成年羊的所有睾丸间质细胞中均检测到P450scc、3βHSD、P450c17和P450arom。在胎儿（90天）和新生儿（15天）的大多数睾丸间质细胞中观察到P450scc和P450c17。在胎儿的一些睾丸间质细胞中发现3βHSD和P450arom，免疫染色较弱，但在新生儿的睾丸间质细胞中，免疫阳性的睾丸间质细胞数量和免疫染色强度增加。这些结果表明，柴山羊睾丸在胎儿、新生儿和成年期均具有合成孕激素、雄激素和雌激素的能力，且这些类固醇激素的合成呈现与年龄相关的增加。