金属诱导环化对寡核苷酸杂交的变构控制。

Allosteric control of oligonucleotide hybridization by metal-induced cyclization.

作者信息

Göritz Mareike, Krämer Roland

机构信息

Anorganisch-Chemisches Institut, Universität Heidelberg, Im Neuenheimer Feld 270, 69120 Heidelberg, Germany.

出版信息

J Am Chem Soc. 2005 Dec 28;127(51):18016-7. doi: 10.1021/ja056463s.

DOI:10.1021/ja056463s

PMID:16366548

Abstract

A bis(terpyridine) modified single-stranded DNA smoothly forms a stable cycle by Fe2+-assisted ring closure. A novel, complex type of allosteric behavior is observed with Zn2+, which reversibly off-regulates binding of a complementary oligonucleotide only in a narrow concentration range.

摘要

双（三联吡啶）修饰的单链DNA通过Fe2+辅助的环化反应顺利形成稳定的环。在Zn2+存在下观察到一种新型的、复杂的变构行为，其仅在狭窄的浓度范围内可逆地下调互补寡核苷酸的结合。