通过改变代谢辅助因子，将光滑球拟酵母中的碳通量从丙酮酸重定向到α-酮戊二酸。

Redirecting carbon flux in Torulopsis glabrata from pyruvate to alpha-ketoglutaric acid by changing metabolic co-factors.

作者信息

Huang H-J, Liu L-M, Li Y, Du G-C, Chen J

机构信息

The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Southern Yangtze University, Wuxi, PR. China.

出版信息

Biotechnol Lett. 2006 Jan;28(2):95-8. doi: 10.1007/s10529-005-4953-1.

DOI:10.1007/s10529-005-4953-1

PMID:16369692

Abstract

The nutrition conditions needed to redirect the carbon flux in Torulopsis glabrata, a pyruvate hyper-production yeast, from pyruvate to alpha-ketoglutaric acid (KG) were investigated in a stirred fermentor. A minor amount of KG (1.3 gl(-1)) was produced when NaOH was used to control the pH, while 12 g KG l(-1) was produced when CaCO(3) was used instead. When thiamine and biotin were included in the medium, 13 g KG l(-1) and 68 g pyruvate l(-1) were produced after 48 h when glucose was nearly consumed (approximately 5 gl(-1)). With fermentation continuing for a further 16 h, the concentration of pyruvate decreased to 31 gl(-1), and KG increased to 30 gl(-1). KG thus accumulated at the expense of pyruvate consumption.

摘要

在搅拌式发酵罐中研究了将光滑球拟酵母（一种丙酮酸高产酵母）中的碳通量从丙酮酸重定向至α-酮戊二酸（KG）所需的营养条件。当使用NaOH控制pH时，产生了少量的KG（1.3 g l⁻¹），而改用CaCO₃时则产生了12 g KG l⁻¹。当培养基中包含硫胺素和生物素时，48 h后葡萄糖几乎消耗殆尽（约5 g l⁻¹）时，产生了13 g KG l⁻¹和68 g丙酮酸l⁻¹。发酵再持续16 h后，丙酮酸浓度降至31 g l⁻¹，而KG增加至30 g l⁻¹。因此，KG以消耗丙酮酸为代价而积累。