Park Mi-Ra, Wang Eun-Hui, Jin Dong-Chan, Cha Jung-Ho, Lee Kweon-Heang, Yang Chul-Woo, Kang Chang-Suk, Choi Yeong-Jin
Department of Clinical Pathology, The Catholic University of Korea, Seoul, Korea.
Proteomics. 2006 Feb;6(3):1066-76. doi: 10.1002/pmic.200500023.
Immunoglobulin A nephropathy (IgAN) is the most common form of immune complex-mediated glomerulonephritis worldwide. Although chronic renal failure develops in considerable numbers of IgAN patients, the exact etiology has not yet been clearly elucidated. To establish the urinary protein map of IgAN, we performed a urinary proteomic analysis. Thirteen patients with IgAN and 12 normal controls were recruited. Morning midstream spot urine samples were used with Centriprep ultrafiltration for concentration and desalting. 2-DE was performed and compared between IgAN and normal control, and urinary proteins were identified by MALDI-TOF MS. A large number of protein spots were identified in IgAN and normal control samples, with means of 311 spots and 174 spots, respectively. Approximately 216 protein spots were detected as differentially expressed in IgAN. Among these, 82 spots were over-expressed, and 134 spots were under-expressed compared to normal controls. A total of 84 differentially expressed spots, representing 59 different proteins, were finally identified in IgAN. We have established a urinary proteomic map of IgAN and this result helps in the identification. Further study is needed to determine the potential pathogenic role of these proteins.
免疫球蛋白A肾病（IgAN）是全球范围内免疫复合物介导的肾小球肾炎最常见的形式。尽管相当数量的IgAN患者会发展为慢性肾衰竭，但其确切病因尚未完全阐明。为了建立IgAN的尿蛋白图谱，我们进行了尿蛋白质组分析。招募了13例IgAN患者和12名正常对照者。使用早晨中段晨尿样本，通过Centriprep超滤进行浓缩和脱盐。进行双向电泳（2-DE）并在IgAN和正常对照之间进行比较，通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定尿蛋白。在IgAN和正常对照样本中鉴定出大量蛋白点，分别平均有311个点和174个点。在IgAN中检测到约216个蛋白点差异表达。其中，与正常对照相比，82个点过度表达，134个点表达不足。最终在IgAN中鉴定出总共84个差异表达点，代表59种不同蛋白质。我们建立了IgAN的尿蛋白质组图谱，这一结果有助于进行鉴定。需要进一步研究以确定这些蛋白质的潜在致病作用。
