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反向囊泡作为一种在有机溶剂中研究酶的新系统。

Reverse vesicles as a new system for studying enzymes in organic solvents.

作者信息

Sánchez-Ferrer A, García-Carmona F

机构信息

Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Murcia, Spain.

出版信息

Biochem J. 1992 Jul 15;285 ( Pt 2)(Pt 2):373-6. doi: 10.1042/bj2850373.

DOI:10.1042/bj2850373
PMID:1637328
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1132796/
Abstract

A new approach to the study of enzyme activity in organic solvents has been developed by using optically transparent reverse vesicles. Polyphenol oxidase was incorporated in an active form into the above ternary system formed by the non-ionic surfactant tetra(ethylene glycol) dodecyl ether/n-dodecane/water. The enzyme in this microenvironment, surprisingly, showed an apparent positive co-operativity which has never before been described either in aqueous solution or in reverse micelles. In addition, the Vmax. expressed was similar to that in water and twice that displayed in reverse micelles.

摘要

通过使用光学透明的反相囊泡，已开发出一种研究有机溶剂中酶活性的新方法。多酚氧化酶以活性形式掺入由非离子表面活性剂四（乙二醇）十二烷基醚/正十二烷/水形成的上述三元体系中。令人惊讶的是，这种微环境中的酶表现出明显的正协同性，这在水溶液或反胶束中均未曾描述过。此外，所表达的Vmax与水中的相似，是反胶束中显示值的两倍。

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