Coulson M L, Hayes N A, Foreman J C
Department of Pharmacology, University College London, UK.
Skin Pharmacol. 1992;5(2):81-6. doi: 10.1159/000211023.
Histamine-induced changes in blood flow in normal human skin were assessed by laser-Doppler velocimetry using 2 lasers of different wavelengths: 780 nm infrared and 633 nm helium-neon. The visible flare response in skin was also measured by planimetry. Laser-Doppler velocimetry was shown to detect both the magnitude of the change in blood flow caused by histamine and also the extent of the changes in the skin. Both parameters were related to the dose of histamine, which ranged between 25 and 750 pmol/site. There was good correlation between the magnitude of the histamine-induced blood flow change and the extent of the response. The flare induced by histamine and measured by planimetry was similar in extent to the blood flow change recorded by laser-Doppler velocimetry. No difference in either the magnitude or the extent of blood flow changes in response to histamine, as measured by laser-Doppler velocimetry, were found when lasers of different wavelengths were used.
采用激光多普勒血流仪，使用两种不同波长的激光（780nm红外激光和633nm氦氖激光）评估组胺引起的正常人皮肤血流变化。皮肤可见的风团反应也通过平面测量法进行测量。结果表明，激光多普勒血流仪既能检测组胺引起的血流变化幅度，也能检测皮肤变化的范围。这两个参数均与组胺剂量相关，组胺剂量范围为25至750pmol/部位。组胺引起的血流变化幅度与反应程度之间存在良好的相关性。通过平面测量法测量的组胺诱导的风团在范围上与激光多普勒血流仪记录的血流变化相似。当使用不同波长的激光时，通过激光多普勒血流仪测量，对组胺反应的血流变化幅度和范围均未发现差异。
