FMRF酰胺相关肽配体激活秀丽隐杆线虫孤儿G蛋白偶联受体Y59H11AL.1。
FMRFamide related peptide ligands activate the Caenorhabditis elegans orphan GPCR Y59H11AL.1.
作者信息
Mertens Inge, Clinckspoor Isabelle, Janssen Tom, Nachman Ronald, Schoofs Liliane
机构信息
Laboratory of Developmental Physiology, Genomics and Proteomics, Katholieke Universiteit Leuven, Naamsestraat 59, Leuven B-3000, Belgium.
出版信息
Peptides. 2006 Jun;27(6):1291-6. doi: 10.1016/j.peptides.2005.11.017. Epub 2005 Dec 27.
G-protein coupled receptors (GPCRs) are ancient molecules that can sense environmental and physiological signals. Currently, the majority of the predicted Caenorhabditis elegans GPCRs are orphan. Here, we describe the characterization of such an orphan C. elegans GPCR, which is categorized in the tachykinin-like group of receptors. Since the C. elegans genome predicts only one tachykinin-like peptide (SFDRMGGTEFGLM), which could not activate the receptor, we hypothesized that one or some of the numerous FMRFamide related peptides (FaRPs) could be the cognate ligands for this receptor. This hypothesis was based on the suggestion that RFamides may be ancestral neuropeptides, from which a lot of the amidated neuropeptides, including tachykinins, derived. Indeed, we found that the orphan receptor encoded by the Y59H11AL.1 gene is activated by several C. elegans neuropeptides, including SPMERSAMVRFamide. These peptides activate the receptor in a concentration-dependent way.
G蛋白偶联受体（GPCRs）是能够感知环境和生理信号的古老分子。目前，大多数预测的秀丽隐杆线虫GPCR都是孤儿受体。在此，我们描述了这样一种孤儿秀丽隐杆线虫GPCR的特征，它被归类于速激肽样受体组。由于秀丽隐杆线虫基因组仅预测了一种速激肽样肽（SFDRMGGTEFGLM），而该肽无法激活该受体，我们推测众多FMRF酰胺相关肽（FaRPs）中的一种或几种可能是该受体的同源配体。这一假设基于以下观点，即RF酰胺可能是祖先神经肽，许多酰胺化神经肽，包括速激肽，都由此衍生而来。事实上，我们发现由Y59H11AL.1基因编码的孤儿受体可被几种秀丽隐杆线虫神经肽激活，包括SPMERSAMVRF酰胺。这些肽以浓度依赖的方式激活该受体。
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