Sturm Sonja, Strasser Eva-Maria, Stuppner Hermann
Institute of Pharmacy, University of Innsbruck, Innrain 52, A-6020 Innsbruck, Austria.
J Chromatogr A. 2006 Apr 21;1112(1-2):331-8. doi: 10.1016/j.chroma.2005.12.008. Epub 2005 Dec 27.
A capillary electrophoresis (CE) method using non-aqueous (NA) separation solutions combined with an ion trap mass spectrometer (MS and MS/MS) as detection device is presented for the separation, identification and quantification of isoquinoline alkaloids from Fumaria officinalis. The best results were obtained with a mixture of acetonitrile-methanol (9:1, v/v) containing 60mM ammonium acetate and 2.2M acetic acid as running electrolyte and an applied voltage of 30 kV. Electrospray MS measurements were performed in the positive ionization mode with isopropanol-water (1:1, v/v) as sheath liquid at a flow rate of 3 microl/min. Alkaloids were detected as M+H-ions and showed typical fragmentation patterns in MS/MS experiments. The developed assay was used for the quantification of seven isoquinoline alkaloids representing different structural subtypes in Fumariae herba extracts and F. herba containing phytopharmaceuticals.
本文介绍了一种毛细管电泳（CE）方法，该方法使用非水（NA）分离溶液，并结合离子阱质谱仪（MS和MS/MS）作为检测装置，用于分离、鉴定和定量来自药用紫堇的异喹啉生物碱。以乙腈 - 甲醇（9:1，v/v）的混合物为最佳结果，该混合物含有60 mM醋酸铵和2.2 M醋酸作为运行电解质，施加电压为30 kV。电喷雾MS测量在正离子化模式下进行，使用异丙醇 - 水（1:1，v/v）作为鞘液，流速为3微升/分钟。生物碱以M + H离子形式被检测到，并在MS/MS实验中显示出典型的碎片模式。所开发的分析方法用于定量代表药用紫堇提取物和含药用紫堇的植物药中不同结构亚型的七种异喹啉生物碱。
