微乳液电动毛细管色谱法分析青霉素的样品堆积

Sample stacking for the analysis of penicillins by microemulsion electrokinetic capillary chromatography.

作者信息

Puig Patricia, Borrull Francesc, Aguilar Carme, Calull Marta

机构信息

Department of Analytical Chemistry and Organic Chemistry, Rovira i Virgili University, Marcel lí Domingo s/n, E-43007 Tarragona, Spain.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Feb 2;831(1-2):196-204. doi: 10.1016/j.jchromb.2005.12.004. Epub 2005 Dec 28.

DOI:10.1016/j.jchromb.2005.12.004

PMID:16386475

Abstract

We present a method for determining eight penicillin antibiotics using microemulsion electrokinetic chromatography (MEEKC). We studied how the composition of the microemulsion affected separation by modifying such parameters as the surfactant or the addition of organic solvents. The best microemulsion system consisted of 0.5% ethyl acetate, 1.2% 1-butanol, 2% Brij 35, 10% 2-butanol and 86.3% 10 mM borate buffer at pH 10. We studied the suitability of this microemulsion composition for analyzing a commercial drug. To improve the sensitivity of the method, we used the stacking technique reversed electrode polarity stacking mode (REPSM), which increased the detection limits by about 40-fold.

摘要

我们提出了一种使用微乳液电动色谱法（MEEKC）测定八种青霉素类抗生素的方法。我们通过改变表面活性剂或添加有机溶剂等参数，研究了微乳液的组成如何影响分离效果。最佳微乳液体系由0.5%乙酸乙酯、1.2%正丁醇、2% Brij 35、10%仲丁醇和86.3% pH值为10的10 mM硼酸盐缓冲液组成。我们研究了这种微乳液组成用于分析市售药物的适用性。为提高该方法的灵敏度，我们使用了堆积技术——反向电极极性堆积模式（REPSM），这使检测限提高了约40倍。