相似文献

1

The RecF protein antagonizes RecX function via direct interaction.

Mol Cell. 2006 Jan 6;21(1):41-50. doi: 10.1016/j.molcel.2005.11.011.

2

SSB antagonizes RecX-RecA interaction.

J Biol Chem. 2008 May 23;283(21):14198-204. doi: 10.1074/jbc.M801511200. Epub 2008 Apr 1.

3

Bacillus subtilis RecA with DprA-SsbA antagonizes RecX function during natural transformation.

Nucleic Acids Res. 2017 Sep 6;45(15):8873-8885. doi: 10.1093/nar/gkx583.

4

RecFOR proteins target RecA protein to a DNA gap with either DNA or RNA at the 5' terminus: implication for repair of stalled replication forks.

J Biol Chem. 2012 Oct 12;287(42):35621-35630. doi: 10.1074/jbc.M112.397034. Epub 2012 Aug 17.

5

A RecA filament capping mechanism for RecX protein.

Mol Cell. 2004 Sep 10;15(5):789-98. doi: 10.1016/j.molcel.2004.08.026.

6

A new insight into RecA filament regulation by RecX from the analysis of conformation-specific interactions.

Elife. 2022 Jun 22;11:e78409. doi: 10.7554/eLife.78409.

7

Direct imaging of RecA nucleation and growth on single molecules of SSB-coated ssDNA.

Nature. 2012 Nov 8;491(7423):274-8. doi: 10.1038/nature11598. Epub 2012 Oct 24.

8

RecA protein filaments: end-dependent dissociation from ssDNA and stabilization by RecO and RecR proteins.

J Mol Biol. 1997 Feb 7;265(5):519-40. doi: 10.1006/jmbi.1996.0748.

9

Protein interactions in genetic recombination in Escherichia coli. Interactions involving RecO and RecR overcome the inhibition of RecA by single-stranded DNA-binding protein.

J Biol Chem. 1994 Nov 25;269(47):30005-13.

10

Complexes of RecA with LexA and RecX differentiate between active and inactive RecA nucleoprotein filaments.

J Mol Biol. 2003 Oct 17;333(2):345-54. doi: 10.1016/j.jmb.2003.08.053.

引用本文的文献

1

Generation and Repair of Postreplication Gaps in Escherichia coli.

Microbiol Mol Biol Rev. 2023 Jun 28;87(2):e0007822. doi: 10.1128/mmbr.00078-22. Epub 2023 May 22.

2

RecF protein targeting to postreplication (daughter strand) gaps I: DNA binding by RecF and RecFR.

Nucleic Acids Res. 2023 Jun 23;51(11):5699-5713. doi: 10.1093/nar/gkad311.

3

Mechanism of RecF-RecO-RecR cooperation in bacterial homologous recombination.

Nat Struct Mol Biol. 2023 May;30(5):650-660. doi: 10.1038/s41594-023-00967-z. Epub 2023 Apr 20.

4

A Robust One-Step Recombineering System for Enterohemorrhagic .

Microorganisms. 2022 Aug 23;10(9):1689. doi: 10.3390/microorganisms10091689.

5

PprA Protein Inhibits DNA Strand Exchange and ATP Hydrolysis of RecA and Regulates the Recombination in Gamma-Irradiated Cells.

Front Cell Dev Biol. 2021 Apr 20;9:636178. doi: 10.3389/fcell.2021.636178. eCollection 2021.

6

Elucidating Recombination Mediator Function Using Biophysical Tools.

Biology (Basel). 2021 Apr 1;10(4):288. doi: 10.3390/biology10040288.

7

A CRISPR-Assisted Nonhomologous End-Joining Strategy for Efficient Genome Editing in Mycobacterium tuberculosis.

mBio. 2020 Jan 28;11(1):e02364-19. doi: 10.1128/mBio.02364-19.

8

Elucidating the functional role of Mycobacterium smegmatis recX in stress response.

Sci Rep. 2019 Jul 29;9(1):10912. doi: 10.1038/s41598-019-47312-3.

9

RecFOR epistasis group: RecF and RecO have distinct localizations and functions in Escherichia coli.

Nucleic Acids Res. 2019 Apr 8;47(6):2946-2965. doi: 10.1093/nar/gkz003.

10

Swi5-Sfr1 stimulates Rad51 recombinase filament assembly by modulating Rad51 dissociation.

Proc Natl Acad Sci U S A. 2018 Oct 23;115(43):E10059-E10068. doi: 10.1073/pnas.1812753115. Epub 2018 Oct 8.

本文引用的文献

1

The DinI and RecX proteins are competing modulators of RecA function.

J Biol Chem. 2004 Dec 31;279(53):55073-9. doi: 10.1074/jbc.M410371200. Epub 2004 Oct 15.

2

Inhibition of RecA protein by the Escherichia coli RecX protein: modulation by the RecA C terminus and filament functional state.

J Biol Chem. 2004 Dec 17;279(51):52991-7. doi: 10.1074/jbc.M409050200. Epub 2004 Oct 4.

3

A RecA filament capping mechanism for RecX protein.

Mol Cell. 2004 Sep 10;15(5):789-98. doi: 10.1016/j.molcel.2004.08.026.

4

Visualization of DNA double-strand break repair in live bacteria reveals dynamic recruitment of Bacillus subtilis RecF, RecO and RecN proteins to distinct sites on the nucleoids.

Mol Microbiol. 2004 Jun;52(6):1627-39. doi: 10.1111/j.1365-2958.2004.04102.x.

5

The DinI protein stabilizes RecA protein filaments.

J Biol Chem. 2004 Jul 16;279(29):30037-46. doi: 10.1074/jbc.M403064200. Epub 2004 May 10.

6

Requirement for RecFOR-mediated recombination in priA mutant.

Mol Microbiol. 2004 Apr;52(2):551-62. doi: 10.1111/j.1365-2958.2004.03997.x.

7

Complexes of RecA with LexA and RecX differentiate between active and inactive RecA nucleoprotein filaments.

J Mol Biol. 2003 Oct 17;333(2):345-54. doi: 10.1016/j.jmb.2003.08.053.

8

The bacterial RecA protein as a motor protein.

Annu Rev Microbiol. 2003;57:551-77. doi: 10.1146/annurev.micro.57.030502.090953.

9

RecFOR proteins load RecA protein onto gapped DNA to accelerate DNA strand exchange: a universal step of recombinational repair.

Mol Cell. 2003 May;11(5):1337-47. doi: 10.1016/s1097-2765(03)00188-6.

10

C-terminal deletions of the Escherichia coli RecA protein. Characterization of in vivo and in vitro effects.

J Biol Chem. 2003 May 2;278(18):16372-80. doi: 10.1074/jbc.M212917200. Epub 2003 Feb 20.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。

RecF蛋白通过直接相互作用拮抗RecX的功能。

The RecF protein antagonizes RecX function via direct interaction.

作者信息

Lusetti Shelley L, Hobbs Michael D, Stohl Elizabeth A, Chitteni-Pattu Sindhu, Inman Ross B, Seifert H Steven, Cox Michael M

机构信息

Department of Biochemistry, University of Wisconsin, Madison, Madison, Wisconsin 53706, USA.

出版信息

Mol Cell. 2006 Jan 6;21(1):41-50. doi: 10.1016/j.molcel.2005.11.011.

DOI:10.1016/j.molcel.2005.11.011

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3894658/

Abstract

The RecX protein inhibits RecA filament extension, leading to net filament disassembly. The RecF protein physically interacts with the RecX protein and protects RecA from the inhibitory effects of RecX. In vitro, efficient RecA filament formation onto single-stranded DNA binding protein (SSB)-coated circular single-stranded DNA (ssDNA) in the presence of RecX occurs only when all of the RecFOR proteins are present. The RecOR proteins contribute only to RecA filament nucleation onto SSB-coated single-stranded DNA and are unable to counter the inhibitory effects of RecX on RecA filaments. RecF protein uniquely supports substantial RecA filament extension in the presence of RecX. In vivo, RecF protein counters a RecX-mediated inhibition of plasmid recombination. Thus, a significant positive contribution of RecF to RecA filament assembly is to antagonize the effects of the negative modulator RecX, specifically during the extension phase.

摘要

RecX蛋白抑制RecA丝的延伸，导致丝的净解聚。RecF蛋白与RecX蛋白发生物理相互作用，并保护RecA免受RecX的抑制作用。在体外，只有当所有RecFOR蛋白都存在时，在RecX存在的情况下，才能在单链DNA结合蛋白（SSB）包被的环状单链DNA（ssDNA）上高效形成RecA丝。RecOR蛋白仅有助于RecA丝在SSB包被的单链DNA上的成核，并且无法对抗RecX对RecA丝的抑制作用。RecF蛋白在RecX存在的情况下独特地支持大量RecA丝的延伸。在体内，RecF蛋白对抗RecX介导的质粒重组抑制。因此，RecF对RecA丝组装的显著正向贡献是拮抗负调节因子RecX的作用，特别是在延伸阶段。