Kaucher Mark S, Harrell William A, Davis Jeffery T
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA.
J Am Chem Soc. 2006 Jan 11;128(1):38-9. doi: 10.1021/ja056888e.
We describe the covalent post-modification of a hydrogen-bonded assembly with the subsequent formation of a potent transmembrane Na+ ion transporter. Olefin metathesis is used to cross-link all 16 guanosine subunits in a lipophilic G-quadruplex. The resulting unimolecular G-quadruplex folds in the environment of a phospholipid membrane and functions as a Na+ ion transporter as judged by fluorescence and 23Na NMR transport assays.
我们描述了氢键组装体的共价后修饰，随后形成了一种高效的跨膜Na⁺离子转运体。烯烃复分解反应用于交联亲脂性G-四链体中的所有16个鸟苷亚基。通过荧光和²³Na NMR转运分析判断，所得的单分子G-四链体在磷脂膜环境中折叠并作为Na⁺离子转运体发挥作用。
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