电喷雾电离过程中磷脂双键的臭氧分解：一种结构测定的新工具。

Ozonolysis of phospholipid double bonds during electrospray ionization: a new tool for structure determination.

作者信息

Thomas Michael C, Mitchell Todd W, Blanksby Stephen J

机构信息

Departments of Chemistry and Biomedical Science, University of Wollongong, Wollongong, New South Wales 2522, Australia.

出版信息

J Am Chem Soc. 2006 Jan 11;128(1):58-9. doi: 10.1021/ja056797h.

DOI:10.1021/ja056797h

PMID:16390120

Abstract

Ozonolysis of double bonds is observed during the negative ion electrospray ionization of unsaturated phospholipids under conditions that produce a corona discharge. Ionic products of the ozonolysis are detected and characterized by mass spectrometry, and the mass-to-charge ratio of these species can be used to unambiguously assign the double bond position within the unsaturated fatty acid radyls. The reaction products are consistent with the gas-phase ozonolysis of desolvated phospholipid ions in an atmosphere rich in volatilized solvent. Reactions may be carried out in a conventional electrospray ionization mass spectrometer and provide a new method for the structural characterization of phospholipids.

摘要

在产生电晕放电的条件下，不饱和磷脂负离子电喷雾电离过程中会观察到双键的臭氧分解。臭氧分解的离子产物通过质谱检测和表征，这些物质的质荷比可用于明确确定不饱和脂肪酸基团内双键的位置。反应产物与富含挥发溶剂的气氛中去溶剂化磷脂离子的气相臭氧分解一致。反应可在传统的电喷雾电离质谱仪中进行，为磷脂的结构表征提供了一种新方法。