内含肽介导的脂化Ras蛋白的体外合成。
Intein-mediated in vitro synthesis of lipidated Ras proteins.
作者信息
Gottlieb D, Grunwald C, Nowak C, Kuhlmann J, Waldmann H
机构信息
Max Planck Institute of Molecular Physiology, Otto-Hahn-Str. 11, 44227, Dortmund, Germany.
出版信息
Chem Commun (Camb). 2006 Jan 21(3):260-2. doi: 10.1039/b511736d. Epub 2005 Nov 28.
DOI:10.1039/b511736d
PMID:16391727
Abstract
Fully functional lipid-modified Ras proteins suitable for the study of Ras-membrane interactions and embodying exclusively native amide bonds can be synthesized in preparative amounts by means of Expressed Protein Ligation.
摘要
通过表达蛋白连接法可以制备出足量的、完全具有功能的脂质修饰的Ras蛋白，该蛋白仅含有天然酰胺键，适用于研究Ras与膜的相互作用。
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