Pirouzpanah Saieed, Rashidi Mohammad Reza, Delazar Abbas, Razavieh Seyyed-Vali, Hamidi Aliasghar
School of Health and Nutrition, Tabriz University of Medical Sciences, Iran.
Chem Pharm Bull (Tokyo). 2006 Jan;54(1):9-13. doi: 10.1248/cpb.54.9.
Ruta graveolens L. is a flavonoid-containing medicinal plant with various biological properties. In the present study, the effects of R. graveolens extract on aldehyde oxidase, a molybdenum hydroxylase, are investigated. Aldehyde oxidase was partially purified from liver homogenates of mature male guinea pigs by heat treatment and ammonium sulphate precipitation. The total extract was obtained by macerating the aerial parts of R. graveolens in MeOH 70% and the effect of this extract on the enzyme activity was assayed using phenanthridine, vanillin and benzaldehyde as substrates. Quercetin and its glycoside form, rutin were isolated, purified and identified from the extract and their inhibitory effects on the enzyme were investigated. R. graveolens extract exhibited a high inhibition on aldehyde oxidase activity (89-96%) at 100 microg/ml which was comparable with 10 microM of menadione, a specific potent inhibitor of aldehyde oxidase. The IC50 values for the inhibitory effect of extract against the oxidation of benzaldehyde, vanillin and phenanthridine were 10.4, 10.1, 43.2 microg/ml, respectively. Both quercetin and rutin at 10 microM caused 70-96% and 27-52% inhibition on the enzyme activity, respectively. Quercetin was more potent inhibitor than rutin, but both flavonols exerted their inhibitory effects mostly in a linear mixed-type.
芸香是一种含有黄酮类化合物的药用植物，具有多种生物学特性。在本研究中，研究了芸香提取物对醛氧化酶（一种钼羟化酶）的影响。通过热处理和硫酸铵沉淀从成年雄性豚鼠的肝脏匀浆中部分纯化醛氧化酶。通过将芸香的地上部分在70%甲醇中浸渍获得总提取物，并以菲啶、香草醛和苯甲醛为底物测定该提取物对酶活性的影响。从提取物中分离、纯化并鉴定了槲皮素及其糖苷形式芦丁，并研究了它们对该酶的抑制作用。芸香提取物在100微克/毫升时对醛氧化酶活性表现出高度抑制（89 - 96%），这与10微摩尔的甲萘醌相当，甲萘醌是醛氧化酶的一种特异性强效抑制剂。提取物对苯甲醛、香草醛和菲啶氧化的抑制作用的IC50值分别为10.4、10.1、43.2微克/毫升。10微摩尔的槲皮素和芦丁分别对酶活性产生70 - 96%和27 - 52%的抑制。槲皮素比芦丁是更有效的抑制剂，但两种黄酮醇大多以线性混合型发挥其抑制作用。
