Hayashi Junya, Takagi Yasushi, Fukuda Hitoshi, Imazato Takayuki, Nishimura Masaki, Fujimoto Motoaki, Takahashi Jun, Hashimoto Nobuo, Nozaki Kazuhiko
Department of Neurosurgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.
J Cereb Blood Flow Metab. 2006 Jul;26(7):906-14. doi: 10.1038/sj.jcbfm.9600247. Epub 2006 Jan 4.
Transplantation of stem cells has the possibility of restoring neural functions after stroke damage. Therefore, we transplanted neuronal progenitors generated from monkey embryonic stem (ES) cells into the ischemic mouse brain to test this possibility. Monkey ES cells were caused to differentiate into neuronal progenitors by the stromal cell-derived inducing activity method. Focal cerebral ischemia was induced by occluding the middle cerebral artery by the intraluminal filament technique. The donor cells were transplanted into the ischemic lateral striatum at 24 h after the start of reperfusion. The cells transplanted into the ischemic brain became located widely around the ischemic area, and, moreover, the transplanted cells differentiated into various types of neurons and glial cells. Furthermore, at 28 days after the transplantation, over 10 times more cells in the graft were labeled with Fluorogold (FG) by stereotactic focal injection of FG into the anterior thalamus and substantia nigra on the grafted side when compared with the number at 14 days. From these results we confirmed the survival and differentiation of, as well as network formation by, monkey ES-cell-derived neuronal progenitors transplanted into the ischemic mouse brain.
干细胞移植有可能在中风损伤后恢复神经功能。因此，我们将猴胚胎干细胞（ES细胞）产生的神经祖细胞移植到缺血性小鼠脑内，以验证这种可能性。采用基质细胞衍生诱导活性法使猴ES细胞分化为神经祖细胞。通过腔内丝线技术闭塞大脑中动脉诱导局灶性脑缺血。在再灌注开始后24小时，将供体细胞移植到缺血侧纹状体。移植到缺血脑内的细胞广泛分布于缺血区域周围，此外，移植细胞分化为各种类型的神经元和胶质细胞。此外，移植后28天时，通过立体定向局灶性注射荧光金（FG）到移植侧的前丘脑和黑质，与14天时相比移植物中被FG标记的细胞数量增加了10倍以上。从这些结果我们证实了移植到缺血性小鼠脑内的猴ES细胞来源的神经祖细胞的存活、分化以及网络形成。
