李痘病毒（PPV）埃尔阿马尔分离株的全基因组序列及其与其他PPV株系的系统发育关系。

The complete genome sequence of an El Amar isolate of plum pox virus (PPV) and its phylogenetic relationship to other PPV strains.

作者信息

Myrta A, Varga A, James D

机构信息

Istituto Agronomico Mediterraneo, Bari, Italy.

出版信息

Arch Virol. 2006 Jun;151(6):1189-98. doi: 10.1007/s00705-005-0703-x. Epub 2006 Jan 9.

DOI:10.1007/s00705-005-0703-x

PMID:16397750

Abstract

The genomic sequence of an El Amar isolate of plum pox virus (PPV) from Egypt was determined by sequencing overlapping cDNA fragments. This is the first complete sequence of a member of the El Amar (EA) strain of PPV. The genome consists of 9791 nt, excluding a poly(A) tail at the 3' terminus. The complete nt sequence of PPV EA is 79-80%, 80%, 77%, and 77% homologous with isolates of strains D/M, Rec (BOR3), C, and W, respectively. The polyprotein identity ranged from 87-91%. Phylogenetic analysis using the complete genome sequence of PPV EA confirmed its strain status. No significant recombination signals were identified using PhylPro and SimPlot scans of the PPV EA sequence, however an interesting recombination signal was identified in the P1/HC-Pro region of PPV W3174.

摘要

通过对重叠cDNA片段进行测序，确定了来自埃及的李痘病毒（PPV）埃尔阿马尔分离株的基因组序列。这是PPV埃尔阿马尔（EA）株系成员的首个完整序列。基因组由9791个核苷酸组成，不包括3'末端的聚腺苷酸尾巴。PPV EA的完整核苷酸序列与D/M、Rec（BOR3）、C和W株系的分离株分别具有79 - 80%、80%、77%和77%的同源性。多聚蛋白的同一性范围为87 - 91%。使用PPV EA的完整基因组序列进行的系统发育分析证实了其株系地位。使用PhylPro和SimPlot对PPV EA序列进行扫描时，未发现明显的重组信号，然而在PPV W3174的P1/HC-Pro区域发现了一个有趣的重组信号。