Allory Yves, Commo Frédéric, Boccon-Gibod Liliane, Sibony Mathilde, Callard Patrice, Ronco Pierre, Debiec Hanna
Institut National de la Santé et de la Recherche Médicale U702 (INSERM), Paris, France.
J Histochem Cytochem. 2006 May;54(5):575-84. doi: 10.1369/jhc.5A6791.2006. Epub 2006 Jan 9.
The HNK-1 carbohydrate epitope is a 3-sulfo-glucuronyl residue attached to lactosamine structures on glycoproteins, proteoglycans, or glycolipids mostly expressed in the nervous system. Here, using monoclonal antibodies against the sulfated HNK-1 carbohydrate epitope, we first examined its distribution in developing and adult kidneys, then its expression in kidneys with tubular necrosis and renal neoplasms. This HNK-1 epitope was expressed in the human, rabbit, and rat, but not mouse kidney. It was detected within a subset of epithelial cells in the renal vesicle and in comma- and S-shaped bodies during early stages of nephrogenesis. In ureteral bud derivatives, the epitope was present transiently in the area where the collecting duct fused with the nephron. In the adult kidney, expression of the HNK-1 epitope became mainly restricted to the thin ascending loop of Henle where this epitope was carried by heparan- and chondro-proteoglycan. In pathological conditions, HNK-1 epitope expression increased dramatically in proximal epithelial tubule cells in kidneys with acute tubular necrosis. In tumors, the HNK-1 epitope was expressed in the epithelial component of nephroblastomas and in a subgroup of papillary renal cell carcinomas. These data suggest that molecules carrying the sulfated HNK-1 carbohydrate epitope may play an important role in critical stages of renal development and in the physiology of thin ascending loop of Henle.
HNK-1碳水化合物表位是一种连接在糖蛋白、蛋白聚糖或糖脂上乳糖胺结构的3-磺酸基葡萄糖醛酸残基，主要在神经系统中表达。在此，我们使用针对硫酸化HNK-1碳水化合物表位的单克隆抗体，首先研究了其在发育中和成年肾脏中的分布，然后研究了其在肾小管坏死和肾肿瘤肾脏中的表达。这种HNK-1表位在人、兔和大鼠肾脏中表达，但在小鼠肾脏中不表达。在肾发生早期，在肾小泡以及逗号状和S形小体的一部分上皮细胞中检测到该表位。在输尿管芽衍生物中，该表位短暂存在于集合管与肾单位融合的区域。在成年肾脏中，HNK-1表位的表达主要局限于髓袢升支细段，该表位由硫酸乙酰肝素和软骨蛋白聚糖携带。在病理情况下，急性肾小管坏死肾脏的近端上皮小管细胞中HNK-1表位表达显著增加。在肿瘤中，HNK-1表位在肾母细胞瘤的上皮成分以及一部分乳头状肾细胞癌中表达。这些数据表明，携带硫酸化HNK-1碳水化合物表位的分子可能在肾脏发育的关键阶段以及髓袢升支细段的生理学中发挥重要作用。
