Zhao Siting, Jin Meilin, Li Hongchao, Tan Yadi, Wang Guihua, Zhang Ruihua, Chen Huanchun
Lab of Animal Infectious Diseases, College of Veterinary Medicine National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China.
Avian Dis. 2005 Dec;49(4):488-93. doi: 10.1637/7321-010405R1.1.
To differentiate avian influenza virus (AIV)-infected chickens vs. chickens immunized with inactivated avian influenza virus, an enzyme-linked immunosorbent assay (ELISA) was developed using a recombinant nonstructural protein (NS1) as the diagnostic antigen, which was cloned from an AIV H9N2 subtype strain isolated during the avian influenza outbreak of 2003-04 and expressed in Escherichia coli. Antibodies to the AIV NS1 protein was only detected in the sera of chickens experimentally infected with AIV but not in the sera of chickens immunized with inactivated vaccine. This ELISA is useful for serological diagnosis to distinguish chickens infected with influenza viruses from those immunized with inactivated vaccine.
为区分感染禽流感病毒（AIV）的鸡与接种了灭活禽流感病毒疫苗的鸡，开发了一种酶联免疫吸附测定（ELISA），该方法使用重组非结构蛋白（NS1）作为诊断抗原，NS1是从2003 - 2004年禽流感暴发期间分离的AIV H9N2亚型毒株中克隆出来的，并在大肠杆菌中表达。仅在经实验感染AIV的鸡血清中检测到针对AIV NS1蛋白的抗体，而在接种灭活疫苗的鸡血清中未检测到。这种ELISA对于血清学诊断区分感染流感病毒的鸡和接种灭活疫苗的鸡很有用。
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