[用原子力显微镜观察次声暴露后L929细胞膜]
[Observation of the L929 cell membrane after infrasound exposure with atomic force microscope].
作者信息
Wang Bing-shui, Chen Jing-zao, Liu Bin, Li Ling, Yi Nan, Liu Jing, Zhang Sa
机构信息
Department of Physiotherapy and Rehabilitation, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China.
出版信息
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2005 Dec;23(6):428-30.
OBJECTIVE
To observe the changes of L929 cell membrane with atomic force microscope (AFM) after infrasound exposure and to explore the mechanisms of effect of infrasound on cell membrane.
METHODS
After primary culture, the L929 cells were exposed to infrasound with intensity output of 130 dB and frequency of 16 Hz 2 hours each day for 3 days. The subsequent changes in the membrane of the control cells and the cells exposed to the infrasound were determined by nano-scale scanning with AFM.
RESULTS
After infrasound exposure, the normal prominence of the membrane became short and the dent became shallow in the 7.5 microm x 7.5 microm and 4.0 microm x 4.0 microm photographs. The prominence appeared as cobblestones. The surface of the membrane became smooth.
CONCLUSION
The membrane structure of the L929 cells can be changed by infrasound exposure with intensity of 130 dB and frequency of 16 Hz. The change might be one of the characteristics of effect of infrasound on cell membrane.
目的
用原子力显微镜（AFM）观察次声暴露后L929细胞膜的变化，探讨次声作用于细胞膜的机制。
方法
原代培养后，将L929细胞每天暴露于强度为130 dB、频率为16 Hz的次声中，每次2小时，共3天。用AFM进行纳米级扫描，测定对照细胞和次声暴露细胞的膜的后续变化。
结果
次声暴露后，在7.5微米×7.5微米和4.0微米×4.0微米的照片中，膜的正常凸起变短，凹陷变浅。凸起呈鹅卵石状。膜表面变得光滑。
结论
强度为130 dB、频率为16 Hz的次声暴露可改变L929细胞的膜结构。这种变化可能是次声作用于细胞膜的特征之一。
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