在病毒包膜蛋白A33R和B5R中发生突变的痘苗病毒在小鼠体内的致病性和免疫原性。

Pathogenicity and immunogenicity in mice of vaccinia viruses mutated in the viral envelope proteins A33R and B5R.

作者信息

Gurt Irina, Abdalrhman Ihab, Katz Ehud

机构信息

Department of Virology, Hebrew University, Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel.

出版信息

Antiviral Res. 2006 Mar;69(3):158-64. doi: 10.1016/j.antiviral.2005.11.006. Epub 2005 Dec 19.

DOI:10.1016/j.antiviral.2005.11.006

PMID:16406098

Abstract

The pathogenicity and immunogenicity in mice of WR.cl and WR.c3, two mutants of the Western Reserve (WR) strain of vaccinia virus, mutated in the A33R and B5R proteins of the outer envelope of the virus, respectively, were studied. WR.c1 was the most attenuated virus, WR.c3 was somewhat more pathogenic, while WR was the most virulent of the three. While the WR and the WR.c3 viruses, intranasally inoculated into mice, spread efficiently to the different internal organs of the animal, including the brain, WR.c1 was restricted to the lungs only. Mice, intranasally infected with 500 plaque forming units of the WR, WR.c1, or WR.c3 viruses, were protected against infection with a lethal dose of the WR strain.

摘要

研究了痘苗病毒西储备（WR）株的两个突变体WR.cl和WR.c3在小鼠中的致病性和免疫原性，这两个突变体分别在病毒外膜的A33R和B5R蛋白中发生了突变。WR.c1是最减毒的病毒，WR.c3的致病性稍强，而WR是三者中毒力最强的。将WR和WR.c3病毒经鼻接种到小鼠体内后，它们能有效地扩散到动物的不同内部器官，包括大脑，而WR.c1仅局限于肺部。经鼻感染500个空斑形成单位的WR、WR.c1或WR.c3病毒的小鼠，可免受致死剂量的WR株感染。