N-乙酰葡糖胺基转移酶V在预后良好的神经母细胞瘤中的高表达：其对细胞凋亡的影响

High expression of N-acetylglucosaminyltransferase V in favorable neuroblastomas: Involvement of its effect on apoptosis.

作者信息

Inamori Kei-ichiro, Gu Jianguo, Ohira Miki, Kawasaki Atsushi, Nakamura Yohko, Nakagawa Takatoshi, Kondo Akihiro, Miyoshi Eiji, Nakagawara Akira, Taniguchi Naoyuki

机构信息

Department of Biochemistry, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.

出版信息

FEBS Lett. 2006 Jan 23;580(2):627-32. doi: 10.1016/j.febslet.2005.12.089. Epub 2006 Jan 5.

DOI:10.1016/j.febslet.2005.12.089

PMID:16406367

Abstract

Neuroblastoma (NBL), derived from the sympathetic precursor cells, is one of the most common pediatric solid tumors. The expression of N-acetylglucosaminyltransferase V and IX (GnT-V and GnT-IX) mRNA in 126 primary NBLs were quantitatively analyzed and higher expression levels of GnT-V were found to be associated with favorable stages (1, 2 and 4s). Conversely, the downregulation of GnT-V expression by small interfering RNA resulted in a decrease in the susceptibility to cell apoptosis induced by retinoic acid in NBL cells accompanied by morphological change. These results suggest that GnT-V is associated with prognosis by modulating the sensitivity of NBLs to apoptosis.

摘要

神经母细胞瘤（NBL）起源于交感神经前体细胞，是最常见的儿科实体瘤之一。对126例原发性神经母细胞瘤中N-乙酰葡糖胺基转移酶V和IX（GnT-V和GnT-IX）mRNA的表达进行了定量分析，发现GnT-V的高表达水平与良好分期（1、2和4s期）相关。相反，小干扰RNA下调GnT-V表达导致神经母细胞瘤细胞对维甲酸诱导的细胞凋亡敏感性降低，并伴有形态学改变。这些结果表明，GnT-V通过调节神经母细胞瘤对凋亡的敏感性与预后相关。