Ban Eunmi, Maeng Jeong-Eun, Woo Jong Soo, Kim Chong-Kook
Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-742, Republic of Korea.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Feb 2;831(1-2):230-5. doi: 10.1016/j.jchromb.2005.12.005. Epub 2006 Jan 10.
A sensitive column-switching high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection was developed for the determination of propiverine in human plasma. Propiverine and internal standard, oxybutynin, were extracted from human plasma that had been made basic with 5N sodium hydroxide into methyl tert-butyl ether. The extracted plasma sample was injected onto the HPLC system consisting of a pretreatment column, a concentrating column, and an analytical column, which were connected with a six-port switching valve. The assay was linear in concentration ranges of 2-200 ng/ml for propiverine in human plasma. This method showed excellent sensitivity (a limit of detection of 0.5 ng/ml), good precision and accuracy. This method is suitable for bioequivalence studies following single dose in healthy volunteers.
建立了一种采用紫外（UV）检测的灵敏的柱切换高效液相色谱（HPLC）法，用于测定人血浆中的丙哌维林。丙哌维林和内标奥昔布宁从用5N氢氧化钠碱化的人血浆中萃取至甲基叔丁基醚中。将萃取后的血浆样品注入由预处理柱、浓缩柱和分析柱组成的HPLC系统，这些柱子通过六通切换阀连接。该测定法在人血浆中丙哌维林浓度范围为2 - 200 ng/ml时呈线性。该方法显示出优异的灵敏度（检测限为0.5 ng/ml）、良好的精密度和准确度。该方法适用于健康志愿者单剂量给药后的生物等效性研究。
