Huang Sheng-Tung, Lin Yuh-Ling
College of Engineering, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taiwan, ROC.
Org Lett. 2006 Jan 19;8(2):265-8. doi: 10.1021/ol052635e.
[reaction: see text] This study describes the design and synthesis of a novel latent fluorophore 3 for DT diaphorase based on the trimethyl lock effect and characterization of its enzymatic kinetics. Fluorophore 3 is also a sensitive fluorimetric reagent for detecting glucose when coupled with DTD and glucose dehydrogenase.
[反应:见正文] 本研究描述了基于三甲基锁定效应设计并合成的一种用于DT黄递酶的新型潜在荧光团3及其酶促动力学特征。荧光团3与DTD和葡萄糖脱氢酶偶联时也是一种检测葡萄糖的灵敏荧光试剂。