Allam J-P, Niederhagen B, Bücheler M, Appel T, Betten H, Bieber T, Bergé S, Novak N
Department of Dermatology, University of Bonn, Bonn, Germany.
Allergy. 2006 Feb;61(2):166-72. doi: 10.1111/j.1398-9995.2005.00965.x.
Mucosal dendritic cells (DC) play a crucial role in tolerance induction as seen in mucosal immunotherapy of atopic diseases. Nevertheless little is known about the phenotypical differences of oral and nasal mucosal DC (nmDC). Recently, we could show that oral mucosal myeloid CD1a(+) DC (omDC) differ from their skin counterparts especially by the expression of high affinity receptor for immunoglobulin E (IgE; FcepsilonRI). However, expression pattern of FcepsilonRI and phenotypical characteristics of CD1a(+) nmDC have not been elucidated in detailed yet.
We performed detailed phenotypical comparison of nmDC and omDC of atopic and nonatopic individuals.
As reported for omDC, FcepsilonRI on nmDC of atopic donors was elevated and mostly occupied by IgE while FcepsilonRI was present only in low amounts on nmDC of nonatopic donors. Nevertheless, the highest FcepsilonRI expression has been observed on omDC. Furthermore, significant amounts of costimulatory molecules CD40, CD80 and CD86 could be detected on nmDC that expressed more CD80 compared with omDC. Moreover, nmDC displayed less major histocompatability complex (MHC) class I and II molecules than omDC. In addition, nmDC expressed more C-type lectins CD205, CD206 as well as myeloid marker CD11b while omDC displayed increased expression of CD207 and lipopolysaccharide (LPS) receptor CD14.
Together these data imply that nmDC phenotypical differ from omDC which might result in diverse functional properties and might be of relevance for selecting routes for immunotherapy of atopic diseases. Moreover these data provide a basis for further studies investigating immunological mechanisms underlying mucosal immunotherapy.
黏膜树突状细胞（DC）在诱导耐受性方面发挥着关键作用，这在特应性疾病的黏膜免疫治疗中可见。然而，关于口腔和鼻黏膜DC（nmDC）的表型差异知之甚少。最近，我们发现口腔黏膜髓样CD1a(+) DC（omDC）与其皮肤对应物不同，特别是通过免疫球蛋白E（IgE；FcepsilonRI）高亲和力受体的表达。然而，FcepsilonRI的表达模式和CD1a(+) nmDC的表型特征尚未详细阐明。
我们对特应性和非特应性个体的nmDC和omDC进行了详细的表型比较。
正如对omDC的报道，特应性供体的nmDC上的FcepsilonRI升高，且大多被IgE占据，而非特应性供体的nmDC上FcepsilonRI仅少量存在。然而，在omDC上观察到最高的FcepsilonRI表达。此外，在nmDC上可检测到大量共刺激分子CD40、CD80和CD86，与omDC相比，nmDC表达更多的CD80。此外，nmDC显示的主要组织相容性复合体（MHC）I类和II类分子比omDC少。另外，nmDC表达更多的C型凝集素CD205、CD206以及髓样标志物CD11b，而omDC显示CD207和脂多糖（LPS）受体CD14的表达增加。
这些数据共同表明nmDC的表型与omDC不同，这可能导致不同的功能特性，并且可能与选择特应性疾病的免疫治疗途径相关。此外，这些数据为进一步研究黏膜免疫治疗的免疫机制提供了基础。
