壳聚糖-DNA纳米粒作为基因载体的研究
[Study on chitosan-DNA nanoparticles as gene carriers].
作者信息
Li Dawei, Zhang Hailing, Ma Jie, Song Liping, Guo Zhiyi, Leng Xigang
机构信息
Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China.
出版信息
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2005 Dec;22(6):1171-6.
The preparation and cell transfection of chitosan-DNA nanoparticles were studied. The TFPI (tissue factor pathway inhibitor) or EGFP (enhanced green fluorescent protein) plasmid DNA was encapsulated with chitosan to form gene nanoparticles. The results with TEM showed that the nanoparticles were of sphere shape. The mean diameter of the nanoparticles was 149 nm and the diameter ranged from 80-250 nm, which were measured by the photo related spectrometry (PCS). The encapsulation efficiency of DNA was 96% +/- 1.38% and the DNA content in the nanoparticles was 37% +/- 3.0%. The encapsulated DNA could be protected from the degradation by DNase I. The transfection efficiency of chitosan nanoparticles were about equivalent to that of the LipofectAMINETM reagent. Our results also showed that chitosan nanoparticles were nontoxic to cultured cells.
研究了壳聚糖-DNA纳米颗粒的制备及细胞转染。将组织因子途径抑制剂（TFPI）或增强型绿色荧光蛋白（EGFP）质粒DNA与壳聚糖包封形成基因纳米颗粒。透射电子显微镜（TEM）结果显示纳米颗粒呈球形。通过光相关光谱法（PCS）测得纳米颗粒的平均直径为149nm，直径范围为80-250nm。DNA的包封率为96%±1.38%，纳米颗粒中的DNA含量为37%±3.0%。包封的DNA可免受DNase I的降解。壳聚糖纳米颗粒的转染效率与LipofectAMINETM试剂相当。我们的结果还表明壳聚糖纳米颗粒对培养细胞无毒。
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