Kim Young-Rok, Paik Hyun-Jong, Ober Christopher K, Coates Geoffrey W, Mark Sonny S, Ryan Thomas E, Batt Carl A
Department of Food Science, 312 Stocking Hall, Cornell University, Ithaca, NY 14853, USA.
Macromol Biosci. 2006 Feb 10;6(2):145-52. doi: 10.1002/mabi.200500213.
The kinetics of enzymatic surface-initiated polymerization of PHB on gold surface has been examined by SPR and the resultant polymer layers characterized by AFM and FT-IR spectrometry. The immobilized enzyme catalyzed surface-initiated polymerization of 3HB-CoA, resulting in the formation of a polymer brush on the surface. The rate of polymer growth from the surface was monitored by SPR in real-time. Polymer growth as measured by the increase in the resonance angle showed no apparent lag phase during the polymerization reaction. SPR analysis also revealed that the thickness of the polymer film could be controlled by varying the initial enzyme density on the surface. The average thicknesses of the PHB film after polymerization reaction were 95, 45 and 15 nm for the surfaces that were treated with 0.5, 0.3 and 0.1*10(-6) M of enzyme, respectively. The binding of PHA synthase at different concentration to the mixed SAMs and subsequent polymerization.
通过表面等离子体共振（SPR）研究了聚羟基丁酸酯（PHB）在金表面的酶促表面引发聚合动力学，并通过原子力显微镜（AFM）和傅里叶变换红外光谱（FT-IR）对所得聚合物层进行了表征。固定化酶催化3-羟基丁酸辅酶A（3HB-CoA）的表面引发聚合反应，在表面形成聚合物刷。通过SPR实时监测聚合物从表面的生长速率。通过共振角的增加测量的聚合物生长在聚合反应过程中没有明显的延迟阶段。SPR分析还表明，可以通过改变表面上初始酶的密度来控制聚合物膜的厚度。对于分别用0.5、0.3和0.1×10⁻⁶ M酶处理的表面，聚合反应后PHB膜的平均厚度分别为95、45和15 nm。不同浓度的聚羟基脂肪酸酯合酶与混合自组装单分子层的结合及随后的聚合反应。
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