采用内控实时聚合酶链反应对粪便样本中产生毒素的艰难梭菌进行快速诊断。
Rapid diagnosis of toxinogenic Clostridium difficile in faecal samples with internally controlled real-time PCR.
作者信息
van den Berg R J, Kuijper E J, van Coppenraet L E S Bruijnesteijn, Claas E C J
机构信息
Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, the Netherlands.
出版信息
Clin Microbiol Infect. 2006 Feb;12(2):184-6. doi: 10.1111/j.1469-0691.2005.01301.x.
A real-time PCR assay for Clostridium difficile was developed, based on the tcdB gene, which detected all known toxinogenic reference strains (n = 45), within 30 serogroups and 24 toxinotypes. The analytical sensitivity was 1 x 10(3) CFU/mL, and the detection limit in faeces was 1 x 10(5) CFU/g. The optimal protocol for DNA extraction from faecal samples involved use of the MagnaPure system with a Stool Transport and Recovery (STAR) buffer pre-treatment. In a 1-month prospective study of 85 patients with diarrhoea, the sensitivity, specificity and positive and negative predictive values of the assay were 100%, 94%, 55% and 100%, respectively, compared with the standard cell cytotoxicity assay.
基于tcdB基因开发了一种艰难梭菌实时PCR检测方法，该方法可检测30个血清群和24个毒素型内的所有已知产毒素参考菌株（n = 45）。分析灵敏度为1×10³ CFU/mL，粪便中的检测限为1×10⁵ CFU/g。从粪便样本中提取DNA的最佳方案包括使用带有粪便转运和回收（STAR）缓冲液预处理的MagnaPure系统。在一项对85例腹泻患者进行的为期1个月的前瞻性研究中，与标准细胞毒性试验相比，该检测方法的灵敏度、特异性以及阳性和阴性预测值分别为100%、94%、55%和100%。
Zotero 插件