Beug Shawn, Vascotto Sandy G, Tsilfidis Catherine
University of Ottawa Eye Institute, Ottawa Health Research Institute, Ottawa, Ontario, Canada.
Dev Dyn. 2006 Mar;235(3):711-22. doi: 10.1002/dvdy.20690.
Red-spotted newts are capable of regenerating various structures and organs through the process of epimorphic regeneration. Receptor tyrosine kinases (RTKs) and their ligands are important for normal cellular development and physiology but most have not yet been characterised during regeneration. We have isolated a newt orthologue of Growth arrest-specific 6 (NvGas6), and examined its expression during forelimb regeneration and within a blastema cell line (B1H1). During limb regeneration, NvGas6 expression increases upon amputation, peaks during maximal blastema cell proliferation, and is subsequently downregulated during redifferentiation. Transcripts are localised to the wound epithelium and distal mesenchymal cells during dedifferentiation and proliferative phases, and scattered within redifferentiating tissues during later stages. In B1H1 cultures, NvGas6 is upregulated under reduced serum conditions and myogenesis. Treatment with mimosine and colchicine or exposure to heat shock or anoxia results in upregulation of NvGas6 expression. Taken together, our findings suggest that during regeneration, NvGas6 expression may be upregulated in response to cellular stress.
红斑蝾螈能够通过形态发生再生过程再生各种结构和器官。受体酪氨酸激酶（RTK）及其配体对正常细胞发育和生理功能很重要，但大多数在再生过程中尚未得到表征。我们分离出了生长停滞特异性6（NvGas6）的蝾螈直系同源物，并研究了其在前肢再生过程中和一个芽基细胞系（B1H1）中的表达情况。在肢体再生过程中，NvGas6的表达在截肢后增加，在芽基细胞最大增殖期间达到峰值，随后在再分化过程中下调。在去分化和增殖阶段，转录本定位于伤口上皮和远端间充质细胞，在后期则分散在再分化组织中。在B1H1培养物中，NvGas6在血清减少条件下和成肌过程中上调。用含羞草碱和秋水仙碱处理或暴露于热休克或缺氧条件下会导致NvGas6表达上调。综上所述，我们的研究结果表明，在再生过程中，NvGas6的表达可能会因细胞应激而上调。
