Takahashi Haruko, Liu Yung-Nan, Liu Hung-Wen
Division of Medicinal Chemistry, College of Pharmacy and Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA.
J Am Chem Soc. 2006 Feb 8;128(5):1432-3. doi: 10.1021/ja0562144.
This report describes a procedure combining six enzymes native to Escherichia coli or Salmonella typhi, such as thymidine kinase (TK), thymidylate kinase (TMK), nucleoside diphosphate kinase (NDK), pyruvate kinase (PK; for ATP regeneration), TDP-glucose synthetase (RfbA), and TDP-glucose 4,6-dehydratase (RfbB), with five enzymes from Streptomyces fradiae, such as TylX3, TylC1, TylC3, TylK, and TylC2, that resulted in the biosynthesis of TDP-l-mycarose from glucose-1-phosphate and thymidine. This two-stage one-pot approach can be readily applied to the synthesis of other unusual sugars.
本报告描述了一种将大肠杆菌或伤寒沙门氏菌的六种天然酶（如胸苷激酶（TK）、胸苷酸激酶（TMK）、核苷二磷酸激酶（NDK）、丙酮酸激酶（PK；用于ATP再生）、TDP-葡萄糖合成酶（RfbA）和TDP-葡萄糖4,6-脱水酶（RfbB））与弗氏链霉菌的五种酶（如TylX3、TylC1、TylC3、TylK和TylC2）相结合的方法，该方法可从1-磷酸葡萄糖和胸苷生物合成TDP-L-鼠李糖。这种两阶段一锅法可轻松应用于其他特殊糖类的合成。
