使用DNA微阵列分析对单纯疱疹病毒1型和2型转录组进行定量比较。

Quantitative comparison of the HSV-1 and HSV-2 transcriptomes using DNA microarray analysis.

作者信息

Aguilar J S, Devi-Rao G V, Rice M K, Sunabe J, Ghazal P, Wagner E K

机构信息

Department of Molecular Biology and Biochemistry and Center for Virus Research, University of California, Irvine, Irvine, CA 92697, USA.

出版信息

Virology. 2006 Apr 25;348(1):233-41. doi: 10.1016/j.virol.2005.12.036. Epub 2006 Jan 31.

DOI:10.1016/j.virol.2005.12.036

PMID:16448680

Abstract

The genomes of human herpes virus type-1 and type-2 share a high degree of sequence identity; yet, they exhibit important differences in pathology in their natural human host as well as in animal host and cell cultures. Here, we report the comparative analysis of the time and relative abundance profiles of the transcription of each virus type (their transcriptomes) using parallel infections and microarray analysis using HSV-1 probes which hybridize with high efficiency to orthologous HSV-2 transcripts. We have confirmed that orthologous transcripts belong to the same kinetic class; however, the temporal pattern of accumulation of 4 transcripts (U(L)4, U(L)29, U(L)30, and U(L)31) differs in infections between the two virus types. Interestingly, the protein products of these transcripts are all involved in nuclear organization and viral DNA localization. We discuss the relevance of these findings and whether they may have potential roles in the pathological differences of HSV-1 and HSV-2.

摘要

人类疱疹病毒1型和2型的基因组具有高度的序列同一性；然而，它们在天然人类宿主、动物宿主和细胞培养物中的病理学表现存在重要差异。在此，我们报告了使用平行感染和微阵列分析对每种病毒类型转录的时间和相对丰度谱（它们的转录组）进行的比较分析，该微阵列分析使用与HSV-2直系同源转录本高效杂交的HSV-1探针。我们已经证实直系同源转录本属于同一动力学类别；然而，4种转录本（U(L)4、U(L)29、U(L)30和U(L)31）的积累时间模式在两种病毒类型的感染中有所不同。有趣的是，这些转录本的蛋白质产物都参与核组织和病毒DNA定位。我们讨论了这些发现的相关性以及它们是否可能在HSV-1和HSV-2的病理差异中发挥潜在作用。