Xu Jing, Hennebold Jon D, Stouffer Richard L
Division of Reproductive Sciences, Oregon National Primate Research Center, Oregon Health and Science University, 505 NW 185th Avenue, Beaverton, OR 97006, USA.
J Clin Endocrinol Metab. 2006 Apr;91(4):1544-53. doi: 10.1210/jc.2005-2776. Epub 2006 Jan 31.
Microarray analysis discovered that mRNA for CRH-binding protein (CRHBP) increased significantly in the primate corpus luteum (CL) after LH withdrawal.
Our objective was to determine whether other components of the CRH/urocortin-receptor-binding protein (UCN-R-BP) system are expressed in the CL during the menstrual cycle and regulated by LH.
CL were collected from monkeys during the early (d 3-5 after the LH surge) to very late (d 18-19) luteal phase and from controls or animals receiving GnRH antagonist (Antide, 3 mg/kg body weight). CRH/UCN-R-BP system components were quantitated for mRNA levels by real-time PCR and analyzed for protein localization by immunohistochemistry.
All genes encoding the CRH/UCN-R-BP system, except for UCN3, were expressed in the CL. CRH mRNA levels did not change during the luteal phase, whereas expression of UCN, UCN2, CRHR1, and CRHR2 was maximal at early or mid luteal phase before declining (P < 0.05) at the later stages. CRHBP mRNA levels were lowest at mid and increased (P < 0.05) in the late luteal phase. Suppressing gonadotropin secretion reduced UCN2 (P < 0.05) and increased CRHBP (P < 0.05) mRNA levels, without altering the expression of other ligands or receptors. CRH, UCN, UCN2 and their receptors were localized to the granulosa-lutein cells of the CL, whereas CRHBP was limited to the theca and theca-lutein cells of the preovulatory follicle and CL.
A local CRH/UCN-R-BP system exists in the macaque CL that is dynamically expressed and LH regulated during the luteal phase of the menstrual cycle. Ligand-receptor activity may regulate luteal structure-function, at this point in an unknown manner, in primates.
微阵列分析发现,促黄体生成素(LH)撤退后,灵长类动物黄体(CL)中促肾上腺皮质激素释放激素结合蛋白(CRHBP)的mRNA显著增加。
我们的目的是确定促肾上腺皮质激素释放激素/尿皮质素-受体结合蛋白(UCN-R-BP)系统的其他成分在月经周期中是否在黄体中表达,并受LH调节。
在黄体期早期(LH峰后第3 - 5天)至晚期(第18 - 19天)从猴子收集黄体,并从对照或接受促性腺激素释放激素拮抗剂(Antide,3 mg/kg体重)的动物收集黄体。通过实时PCR定量CRH/UCN-R-BP系统成分的mRNA水平,并通过免疫组织化学分析蛋白质定位。
除UCN3外,所有编码CRH/UCN-R-BP系统的基因均在黄体中表达。黄体期CRH mRNA水平未发生变化,而UCN、UCN2、CRHR1和CRHR2的表达在黄体早期或中期达到最大值,随后在后期下降(P < 0.05)。CRHBP mRNA水平在中期最低,在黄体晚期升高(P < 0.05)。抑制促性腺激素分泌可降低UCN2(P < 0.05)并增加CRHBP(P < 0.05)的mRNA水平,而不改变其他配体或受体的表达。CRH、UCN、UCN2及其受体定位于黄体的颗粒黄体细胞,而CRHBP仅限于排卵前卵泡和黄体的卵泡膜及卵泡膜黄体细胞。
猕猴黄体中存在局部CRH/UCN-R-BP系统,该系统在月经周期的黄体期动态表达并受LH调节。在灵长类动物中,配体-受体活性可能以未知方式调节黄体的结构功能。