Yang Jehn-Hsiahn, Wu Ming-Yih, Chang Daw-Yuan, Chang Chien-Huei, Yang Yu-Shih, Ho Hong-Nerng
Department of Obstetrics and Gynecology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipie, Taiwan.
Am J Reprod Immunol. 2006 Mar;55(3):181-7. doi: 10.1111/j.1600-0897.2005.00363.x.
To determine the effects of macrophage on endometrial stromal cells (ESCs) in women with adenomyosis.
Eutopic endometrium was obtained and separated into single ESC in 10 women with adenomyosis (study group) and 11 without adenomyosis (control group). ESCs were then cultured alone or with macrophage for 24 hr.
Immunohistochemistry identified the presence of interleukin-6 (IL-6), IL-8, and IL-10 in ESCs. Real-time quantitative PCR revealed that the IL-6 mRNA was significantly expressed in macrophage-cocultured ESCs in adenomyosis than that in the controls, but was not different in ESCs cultured alone between the two groups. The levels of IL-8 and IL-10 mRNA were similar in ESCs either cultured alone or with macrophage between women with and without adenomyosis.
IL-6 mRNA was significantly expressed in ESCs after in vitro coculture with macrophage in adenomyosis. This aberrant behavior of ESCs might play a role in the formation of ectopic endometrial implants in adenomyosis.
确定巨噬细胞对子宫腺肌病患者子宫内膜基质细胞（ESCs）的影响。
获取10例子宫腺肌病患者（研究组）和11例非子宫腺肌病患者（对照组）的在位内膜，并将其分离为单个ESC。然后将ESCs单独培养或与巨噬细胞共培养24小时。
免疫组织化学鉴定出ESCs中存在白细胞介素-6（IL-6）、IL-8和IL-10。实时定量PCR显示，与对照组相比，子宫腺肌病患者与巨噬细胞共培养的ESCs中IL-6 mRNA显著表达，但两组单独培养的ESCs中无差异。有或无子宫腺肌病的女性中，单独培养或与巨噬细胞共培养的ESCs中IL-8和IL-10 mRNA水平相似。
子宫腺肌病患者的ESCs与巨噬细胞体外共培养后，IL-6 mRNA显著表达。ESCs的这种异常行为可能在子宫腺肌病异位子宫内膜植入物的形成中起作用。
Zotero 插件