Sellin Julia, Albrecht Stefanie, Kölsch Verena, Paululat Achim
Universität Osnabrück, Fachbereich Biologie/Chemie - Zoologie, Barbarastrasse 11, 49069 Osnabrück, Germany.
Gene Expr Patterns. 2006 Apr;6(4):360-75. doi: 10.1016/j.modgep.2005.09.012. Epub 2006 Feb 7.
Drosophila melanogaster has become one of the important model systems to investigate the development and differentiation of the heart. After 24h after egg deposition (h AED), a simple tube-like organ is formed, consisting of essentially only two cell types, the contractile cardioblasts and non-myogenic pericardial cells. In contrast to the detailed knowledge of heart formation during embryogenesis, only a few studies deal with later changes in heart morphology and/or function. This is mainly due to the difficulties to carry out whole mount stainings in later stages without complicated dissections or treatments of the cuticle and puparium. In this paper we describe the identification of a hand genomic region, which is fully sufficient to drive GFP expression in heart cells of embryos, larvae, and adults. This serves as an initial step to understand the position of hand in the early regulatory network in heart development. Furthermore, we demonstrate that our newly created GFP reporter line is extremely useful to study postembryonic heart differentiation. For the first time we document heart differentiation in living animals throughout all developmental stages of Drosophila melanogaster, including embryogenesis, all three larval stages, metamorphosis, and the adult life with respect to pericardial cells and cardiomyocytes.
黑腹果蝇已成为研究心脏发育和分化的重要模型系统之一。在产卵后24小时（h AED），形成了一个简单的管状器官，基本上仅由两种细胞类型组成，即收缩性成心肌细胞和非肌源性心包细胞。与胚胎发育过程中心脏形成的详细知识相比，只有少数研究涉及心脏形态和/或功能的后期变化。这主要是由于在后期阶段进行整体染色而不进行复杂的解剖或角质层和蛹壳处理存在困难。在本文中，我们描述了一个hand基因组区域的鉴定，该区域足以驱动胚胎、幼虫和成虫心脏细胞中的绿色荧光蛋白（GFP）表达。这是了解hand在心脏发育早期调控网络中位置的第一步。此外，我们证明我们新创建的GFP报告系对于研究胚胎后心脏分化非常有用。我们首次记录了黑腹果蝇在所有发育阶段（包括胚胎发生、所有三个幼虫阶段、变态和成虫期）中心包细胞和心肌细胞的活体心脏分化情况。
