Zhang Xiaoyu, Wu Huifeng, Liao Peiqiu, Li Xiaojing, Ni Jiazuan, Pei Fengkui
Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of Chinese Academy of Sciences, Changchun 130022, PR China.
Food Chem Toxicol. 2006 Jul;44(7):1006-14. doi: 10.1016/j.fct.2005.12.004. Epub 2006 Feb 2.
The subacute toxicity of aristolochic acid (AA) was investigated by (1)H NMR spectroscopic and pattern recognition (PR)-based metabonomic methods. Model toxins were used to enable comparisons of the urinary profiles from rats treated with known toxicants and AA at various time intervals. Urinary (1)H NMR spectra were data-processed and analyzed by pattern recognition method. The result of visual comparison of the spectra showed that AA caused a renal proximal tubular and papillary lesion and a slight hepatic impair. Pattern recognition analysis indicated that the renal proximal tubule lesion was the main damage induced by AA, and the renal toxicity induced by AA was a progressive course with the accumulation of dosage by monitoring the toxicological processes from onset, development and part-recovery. These results were also supported by the conventional clinical biochemical parameters.
通过基于氢核磁共振（¹H NMR）光谱和模式识别（PR）的代谢组学方法研究了马兜铃酸（AA）的亚急性毒性。使用模型毒素对已知毒物和不同时间间隔AA处理的大鼠尿液谱进行比较。尿液¹H NMR光谱经数据处理后用模式识别方法进行分析。光谱的视觉比较结果表明，AA导致肾近端小管和乳头病变以及轻微的肝脏损伤。模式识别分析表明，肾近端小管病变是AA诱导的主要损伤，通过监测从发病、发展到部分恢复的毒理学过程，AA诱导的肾毒性是一个随着剂量积累的渐进过程。这些结果也得到了传统临床生化参数的支持。
