从噬菌体肽库中筛选出的GD3模拟肽可诱导产生GD3神经节苷脂抗体反应。

GD3-replica peptides selected from a phage peptide library induce a GD3 ganglioside antibody response.

作者信息

Popa Iuliana, Ishikawa Dai, Tanaka Michinori, Ogino Koichi, Portoukalian Jacques, Taki Takao

机构信息

INSERM U.346, Edouard Herriot Hospital, Lyon, France.

出版信息

FEBS Lett. 2006 Feb 20;580(5):1398-404. doi: 10.1016/j.febslet.2006.01.063. Epub 2006 Jan 30.

DOI:10.1016/j.febslet.2006.01.063

PMID:16458892

Abstract

GD3-replica peptides were obtained from a phage peptide library and an anti-GD3 monoclonal antibody (Mab) (4F6), and anti-GD3 Mabs were generated by immunizing a peptide GD3P4. A Mab, 3D2 was found to recognize GD3 by immunohistochemical approaches. Amino acid analysis of heavy and light chain variable regions of 4F6 and 3D2 showed that the respective chains had the same length, and only a few different amino acid substitutions were found. The present data indicate that the immunogenic GD3P4 is processed in a certain size and exposed on the antigen-presenting cells with a molecular shape quite similar to that of the GD3 epitope in 4F6.

摘要

GD3模拟肽是从噬菌体肽库中获得的，还有一种抗GD3单克隆抗体（Mab）（4F6），抗GD3单克隆抗体是通过用肽GD3P4免疫产生的。通过免疫组织化学方法发现一种单克隆抗体3D2可识别GD3。对4F6和3D2重链和轻链可变区的氨基酸分析表明，各自的链长度相同，仅发现少数不同的氨基酸替换。目前的数据表明，具有免疫原性的GD3P4以一定大小被加工处理，并以与4F6中GD3表位分子形状非常相似的形式暴露于抗原呈递细胞上。