Agis H, Krauth M-T, Mosberger I, Müllauer L, Simonitsch-Klupp I, Schwartz L B, Printz D, Böhm A, Fritsch G, Horny H-P, Valent P
Department of Internal Medicine I, Division of Haematology and Haemostaseology, Medical University of Vienna, Austria.
J Clin Pathol. 2006 Apr;59(4):396-402. doi: 10.1136/jcp.2005.029215. Epub 2006 Feb 3.
Basophils are highly specialised granulocytes that express a unique profile of antigens and increase in myeloproliferative disorders (MPD). In chronic myeloid leukaemia (CML), basophilia is a diagnostic and prognostic determinant. So far, however, no reliable approach for routine detection and enumeration of bone marrow basophils has become available.
To detect and enumerate basophils in bone marrow sections in patients with CML and other MPD.
The anti-basophil antibody 2D7 was applied to paraffin embedded bone marrow sections from normal/reactive subjects (n = 31), patients with CML (chronic phase, n = 37; accelerated phase, n = 9), and other MPD (chronic idiopathic myelofibrosis (CIMF), n = 20; polycythaemia vera (PV), n = 20; essential thrombocythaemia (ET), n = 20; indolent systemic mastocytosis (ISM), n = 7).
As assessed by serial section staining, 2D7(+) cells were found to co-express myeloperoxidase, histidine decarboxylase, CD9, and CD43, but did not express B cell or T cell restricted antigens. 2D7(+) bone marrow cells were found to increase in CML compared with normal/reactive bone marrow and other MPD (median numbers of 2D7(+) cells/mm(2): CML, 33; normal/reactive bone marrow, 6; CIMF, 10; PV, 6; ET, 5; ISM, 3; p<0.05). The highest basophil counts were recorded in accelerated phase CML (115/mm(2)).
A novel immunohistochemical procedure has been established for basophil detection in normal bone marrow and MPD. This approach should help in the quantification of bone marrow basophils at diagnosis and during anti-leukaemic treatment.
嗜碱性粒细胞是高度特化的粒细胞，表达独特的抗原谱，且在骨髓增殖性疾病（MPD）中数量增加。在慢性髓性白血病（CML）中，嗜碱性粒细胞增多是一个诊断和预后的决定因素。然而，迄今为止，尚无可靠的方法可用于常规检测和计数骨髓嗜碱性粒细胞。
检测和计数CML及其他MPD患者骨髓切片中的嗜碱性粒细胞。
将抗嗜碱性粒细胞抗体2D7应用于来自正常/反应性受试者（n = 31）、CML患者（慢性期，n = 37；加速期，n = 9）以及其他MPD患者（慢性特发性骨髓纤维化（CIMF），n = 20；真性红细胞增多症（PV），n = 20；原发性血小板增多症（ET），n = 20；惰性系统性肥大细胞增多症（ISM），n = 7）的石蜡包埋骨髓切片。
通过连续切片染色评估，发现2D7(+)细胞共表达髓过氧化物酶、组氨酸脱羧酶、CD9和CD43，但不表达B细胞或T细胞限制性抗原。与正常/反应性骨髓及其他MPD相比，发现CML中2D7(+)骨髓细胞增多（2D7(+)细胞/mm²的中位数：CML为33；正常/反应性骨髓为6；CIMF为10；PV为6；ET为5；ISM为3；p<0.05）。CML加速期的嗜碱性粒细胞计数最高（115/mm²）。
已建立一种用于正常骨髓和MPD中嗜碱性粒细胞检测的新型免疫组织化学方法。该方法应有助于在诊断时及抗白血病治疗期间对骨髓嗜碱性粒细胞进行定量分析。
