Howlader Gitanjali, Paranjpe Dhanashree A, Sharma Vijay Kumar
Chronobiology Laboratory, Evolutionary and Organismal Biology Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India.
J Biol Rhythms. 2006 Feb;21(1):13-20. doi: 10.1177/0748730405282882.
The authors report the results of their study aimed at investigating the consequence of targeted ablation of ventral lateral neurons (LN(v)s--neurons regulating eclosion and locomotor activity rhythms) and genetic disruption of pigment-dispersing factor (PDF--an important output of circadian clocks) on the egg-laying rhythm of Drosophila melanogaster. The results clearly suggest that genetic ablation of LN(v)s and loss of function mutation of PDF abolish eclosion and locomotor activity rhythms, whereas the egg-laying rhythm continues unabated. Furthermore, the results also demonstrate that the period of egg-laying rhythm remains unchanged under different ambient temperatures and nutrition levels, suggesting that the egg-laying rhythm of D. melanogaster is temperature and nutrition compensated. Based on these results, the authors conclude that the egg-laying rhythm in D. melanogaster is regulated by non-LN(v)-based, non-PDF-mediated circadian clocks.
作者报告了他们的研究结果，该研究旨在调查腹外侧神经元（LN(v)s，即调节羽化和运动活动节律的神经元）的靶向消融以及色素分散因子（PDF，昼夜节律钟的重要输出）的基因破坏对黑腹果蝇产卵节律的影响。结果清楚地表明，LN(v)s的基因消融和PDF的功能丧失突变消除了羽化和运动活动节律，而产卵节律却不受影响地持续存在。此外，结果还表明，在不同的环境温度和营养水平下，产卵节律的周期保持不变，这表明黑腹果蝇的产卵节律是温度和营养补偿性的。基于这些结果，作者得出结论，黑腹果蝇的产卵节律是由基于非LN(v)、非PDF介导的昼夜节律钟调节的。
