干扰素-γ诱导Huh 6细胞上HLA I类抗原表达的信号转导途径。
Signal transduction pathways in the induction of HLA class I antigen expression on Huh 6 cells by interferon-gamma.
作者信息
Towata T, Hayashi N, Katayama K, Takehara T, Sasaki Y, Kasahara A, Fusamoto H, Kamada T
机构信息
First Department of Medicine, Osaka University Medical School, Japan.
出版信息
Biochem Biophys Res Commun. 1991 Jun 14;177(2):610-8. doi: 10.1016/0006-291x(91)91832-w.
This study investigated the intracellular signal transduction regulating the appearance of HLA class I antigens on Huh 6 cells induced by interferon-gamma. The expression was blocked by a protein kinase C inhibitor, H-7, but not by a calmodulin antagonist, W-7, nor by a protein kinase A inhibitor, H-8, at low dose. The antigen expression was induced by a direct activator of protein kinase C, phorbol myristate acetate, but not by calcium ionophore A23187 nor an analog of cAMP, dbcAMP. Therefore, we concluded that protein kinase C is involved in the expression of HLA class I antigens on Huh 6 cells induced by interferon-gamma but Ca(2+)-calmodulin and cAMP are not.
本研究调查了调节γ干扰素诱导的Huh 6细胞上HLA I类抗原出现的细胞内信号转导。低剂量时，该表达被蛋白激酶C抑制剂H-7阻断，但未被钙调蛋白拮抗剂W-7或蛋白激酶A抑制剂H-8阻断。蛋白激酶C的直接激活剂佛波醇肉豆蔻酸酯乙酸盐可诱导抗原表达，但钙离子载体A23187或cAMP类似物二丁酰环磷腺苷酸不能诱导。因此，我们得出结论，蛋白激酶C参与γ干扰素诱导的Huh 6细胞上HLA I类抗原的表达，而Ca(2+)-钙调蛋白和cAMP不参与。
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