Weiping Sui, Changqing Yin, Yanjing Chen, Zhiguo Zhang, Xiangzheng Kong
School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Colloids Surf B Biointerfaces. 2006 Mar 1;48(1):13-6. doi: 10.1016/j.colsurfb.2006.01.005. Epub 2006 Feb 8.
A kind of amphiphilic derivatives of chitosan (2-hydroxyl-3-butoxyl)-propylcarboxymethyl-chitosan (HBP-CMCHS), has been synthesized, and the critical micelle concentration (cmc) of HBP-CMCHS was detected by the fluorescence method. The puerarin-loaded HBP-CMCHS micellar system was prepared by physical entrapped method. Result showed that when adding the same amount of puerarin, the solubilizing capacity was enhanced by increasing the concentration of HBP-CMCHS and temperature. Puerarin-loaded micellar system of HBP-CMCHS was characterized by TEM and DLS. TEM photograph revealed that the micelles were spherical and puerarin was solubilized in the cores of the spherical polymeric micelles. DLS showed that after solubilization the size of the micelles became bigger. In vitro tests showed that puerarin was slowly released from micellar solution and the release lasted up to 60 h by means of the dialysis method.
合成了一种壳聚糖两亲性衍生物（2-羟基-3-丁氧基）-丙基羧甲基壳聚糖（HBP-CMCHS），并采用荧光法测定了HBP-CMCHS的临界胶束浓度（cmc）。通过物理包埋法制备了载有葛根素的HBP-CMCHS胶束体系。结果表明，加入等量葛根素时，通过提高HBP-CMCHS浓度和温度，增溶能力增强。采用透射电子显微镜（TEM）和动态光散射（DLS）对载有葛根素的HBP-CMCHS胶束体系进行了表征。TEM照片显示，胶束呈球形，葛根素溶解在球形聚合物胶束的核中。DLS表明，溶解后胶束尺寸变大。体外试验表明，葛根素从胶束溶液中缓慢释放，通过透析法释放持续长达60小时。
