Jung In-Gyung, Park Ok-Hyun
Department of Environmental Engineering, Pusan National University, Gumjung-gu, Korea.
J Biosci Bioeng. 2005 Dec;100(6):657-61. doi: 10.1263/jbb.100.657.
A biofilter column inoculated with Pseudomonas putida F1 was operated to study cometabolic biodegradation of trichloroethylene (TCE) gas using toluene as a primary substrate. Variations in the efficiency and capacity of TCE elimination with different inlet concentrations of toluene and TCE were investigated in order to understand the competitive inhibition between toluene and TCE. Two toluene feeding methods, stage feeding along the column and cyclic feeding, were examined as strategies to enhance TCE cometabolic biodegradation by avoiding the toluene inhibition of TCE biodegradation and the toxic effect of TCE on cells and toluene dioxygenase enzymes. It was concluded that both methods are promising and that the determination of a suitable feeding frequency, recovery period, and inlet toluene concentration was required to optimize cyclic feeding in the cometabolic biodegradation of TCE.
运行一个接种恶臭假单胞菌F1的生物滤池柱,以甲苯作为主要底物研究三氯乙烯(TCE)气体的共代谢生物降解。研究了不同甲苯和TCE进口浓度下TCE去除效率和容量的变化,以了解甲苯和TCE之间的竞争性抑制作用。考察了两种甲苯进料方式,即沿柱分段进料和循环进料,作为避免甲苯对TCE生物降解的抑制以及TCE对细胞和甲苯双加氧酶的毒性作用来增强TCE共代谢生物降解的策略。得出的结论是,这两种方法都很有前景,并且需要确定合适的进料频率、恢复期和进口甲苯浓度,以优化TCE共代谢生物降解中的循环进料。