来自嗜热自养甲烷杆菌（菌株δH）的F390合成酶和F390水解酶。
F390 synthetase and F390 hydrolase from Methanobacterium thermoautotrophicum (strain delta H).
作者信息
Kengen S W, von den Hoff H W, Keltjens J T, van der Drift C, Vogels G D
机构信息
Department of Microbiology, Faculty of Science, University of Nijmegen, The Netherlands.
出版信息
Biofactors. 1991 Jan;3(1):61-5.
Factor F390 is the 8-OH adenylated form of the deazaflavin coenzyme F420, which is a central electron carrier in methanogenic bacteria. The enzymes catalysing the formation of F390 from ATP and F420 (F390 synthetase) and its hydrolysis into AMP and F420 (F390 hydrolase) were isolated and partially purified from Methanobacterium thermoautotrophicum. Both enzymes were oxygen-stable. The F390 synthetase tended to coelute with coenzyme F420 reducing hydrogenase during all purification steps. The 30-fold purified enzyme was still contaminated with the hydrogenase. The F390 hydrolase was purified 135-fold to a specific activity of 8.6 mumol/min/mg protein. The colourless enzyme consisted of one polypeptide of approximately 27,000 kd.
因子F390是脱氮黄素辅酶F420的8-羟基腺苷化形式，F420是产甲烷菌中的一种核心电子载体。从嗜热自养甲烷杆菌中分离并部分纯化了催化由ATP和F420形成F390的酶（F390合成酶）及其水解为AMP和F420的酶（F390水解酶）。这两种酶对氧气均稳定。在所有纯化步骤中，F390合成酶倾向于与辅酶F420还原氢化酶共洗脱。经过30倍纯化的酶仍被氢化酶污染。F390水解酶被纯化了135倍，比活性达到8.6 μmol/分钟/毫克蛋白质。这种无色酶由一条约27,000 kd的多肽组成。
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