Neves Adriana Pires, Keller Andrea, Trein Cristina Rodrigues, Möller Gabriella, Jobim Maria Inês Mascarenhas, Castilho Luis Fernando Fiori, Cardoso Marisa Ribeiro de Itapema, Leibold Wolfgang, Zerbe Holm, Klug Erich, Gregory Ricardo Macedo, Mattos Rodrigo Costa
REPROLAB, Departamento de Medicina Animal, FAVET, UFRGS, Av. Bento Gonçalves 9090, 91540-000 Porto Alegre, RS, Brazil.
Anim Reprod Sci. 2007 Feb;97(3-4):314-22. doi: 10.1016/j.anireprosci.2006.01.010. Epub 2006 Feb 20.
This study compared four treatments for bacterial endometritis in mares experimentally infected with Streptococcus zooepidemicus. Twenty-five mares were used, 20 resistant and five susceptible to endometritis. Mares would be in estrus when infected. Twenty-four hours after inoculation, clinical, bacteriological and cytological examinations were performed and repeated until the first occurrence: negative cytology and no Streptococcus growth or the seventh day post-infection. All mares showed clinical signs of endometritis and were assigned to one of the following treatments: (1) intrauterine infusion of fresh leukocytes; (2) intrauterine infusion of frozen-thawed leukocytes; (3) intrauterine infusion of lysed leukocytes; (4) intrauterine infusion of recombinant human interleukin-8 (rhIL-8); (5) control. Mares were submitted to all treatments, with at least a 14-day interval between treatments in a Latin square design. Treatment did not affect (P=0.121) time needed for resistant mares to eliminate bacteria. Time needed for elimination of bacteria was similar in susceptible mares treated with fresh and frozen leukocytes (P=0.333). Susceptible mares treated with frozen leukocytes also did not differ from those treated with lysed leukocytes (P=0.227) for time to eliminate bacteria, but were significantly different (P>0.02) from those treated with rhIL-8 and control. In resistant mares, physical clearance ability was probably the responsible for bacterial elimination. Intrauterine infusions in susceptible mares with viable or lysed leukocytes associated or not to opsonizing factors, reduced the time to elimination of bacteria. Infusions with bactericidal effect (functional neutrophils and granules) was likely effective and responsible for the more rapid elimination of bacteria in susceptible mares.
本研究比较了四种治疗实验性感染马链球菌的母马细菌性子宫内膜炎的方法。使用了25匹母马，其中20匹对子宫内膜炎有抵抗力，5匹易感。母马感染时处于发情期。接种后24小时进行临床、细菌学和细胞学检查，并重复进行直至首次出现以下情况：细胞学检查为阴性且无马链球菌生长，或感染后第7天。所有母马均表现出子宫内膜炎的临床症状，并被分配到以下治疗组之一：（1）子宫内注入新鲜白细胞；（2）子宫内注入冻融白细胞；（3）子宫内注入裂解白细胞；（4）子宫内注入重组人白细胞介素-8（rhIL-8）；（5）对照组。母马接受了所有治疗，采用拉丁方设计，治疗之间至少间隔14天。治疗对有抵抗力的母马清除细菌所需时间没有影响（P=0.121）。用新鲜和冻融白细胞治疗的易感母马清除细菌所需时间相似（P=0.333）。用冻融白细胞治疗的易感母马在清除细菌的时间上与用裂解白细胞治疗的母马也没有差异（P=0.227），但与用rhIL-8和对照组治疗的母马有显著差异（P>0.02）。在有抵抗力的母马中，物理清除能力可能是清除细菌的原因。在易感母马子宫内注入活的或裂解的白细胞，无论是否与调理因子相关，都减少了清除细菌的时间。具有杀菌作用的注入物（功能性中性粒细胞和颗粒）可能有效，并导致易感母马中细菌更快清除。
