Wang Jin, He Fang-ting, Tzang Chi-hung, Fong Wang-fan, Xiao Pei-gen, Han Rui, Yang Meng-su
Applied Research Center for Genomics Technology, Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China.
Yao Xue Xue Bao. 2005 Dec;40(12):1099-104.
To elucidate the molecular mechanism of cell cycle arrest and apoptosis of MCF-7 cells induced by paclitaxel.
Flow cytometry was used to determine the cell cycle changes of MCF-7 cells upon paclitaxel treatment. Gene expression profiles of MCF-7 cells induced by paclitaxel were obtained by using cDNA microarrays containing 9984 genes and expressed sequence tags (ESTs).
Cell cycle analysis showed that 77.8% of cells arrested at G2/M phase and 1.3% of cells underwent apoptosis upon 100 nmol x L(-1) paclitaxel treatment for 24 hours; cDNA microarray results revealed that 27 and 77 genes were differentially expressed upon 12.5 nmol x L(-1) (IC50) and 100 nmol x L(-1) paclitaxel treatment, respectively.
Paclitaxel stabilized microtubules and caused G2/M cell cycle arrest and apoptotic cell death in a concentration-dependent manner, which is associated with the regulation of selected genes related to microtubule assembly and cytoskeleton, cell cycle regulation, and DNA repair and apoptosis.
阐明紫杉醇诱导MCF - 7细胞周期阻滞和凋亡的分子机制。
采用流式细胞术测定紫杉醇处理后MCF - 7细胞的细胞周期变化。利用包含9984个基因和表达序列标签（ESTs）的cDNA微阵列获得紫杉醇诱导的MCF - 7细胞的基因表达谱。
细胞周期分析表明，100 nmol·L⁻¹紫杉醇处理24小时后，77.8%的细胞阻滞于G2/M期，1.3%的细胞发生凋亡；cDNA微阵列结果显示，12.5 nmol·L⁻¹（IC50）和100 nmol·L⁻¹紫杉醇处理后分别有27个和77个基因差异表达。
紫杉醇稳定微管并以浓度依赖的方式导致G2/M期细胞周期阻滞和凋亡性细胞死亡，这与微管组装和细胞骨架、细胞周期调控以及DNA修复和凋亡相关的特定基因的调节有关。
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