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2
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本文引用的文献

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A peripheral element assembles the compact core structure essential for group I intron self-splicing.一个外围元件组装了I组内含子自我剪接所必需的紧密核心结构。
Nucleic Acids Res. 2005 Aug 12;33(14):4602-11. doi: 10.1093/nar/gki770. Print 2005.
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Predicting the secondary structures and tertiary interactions of 211 group I introns in IE subgroup.预测IE亚组中211个I类内含子的二级结构和三级相互作用。
Nucleic Acids Res. 2005 Apr 20;33(7):2118-28. doi: 10.1093/nar/gki517. Print 2005.
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Metal ions and RNA folding: a highly charged topic with a dynamic future.金属离子与RNA折叠：一个充满活力且前景广阔的热门话题。
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Crystal structure of a phage Twort group I ribozyme-product complex.噬菌体Twort I组核酶-产物复合物的晶体结构
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5
Fast formation of the P3-P7 pseudoknot: a strategy for efficient folding of the catalytically active ribozyme.P3-P7假结的快速形成：催化活性核酶高效折叠的一种策略。
RNA. 2005 Jan;11(1):59-69. doi: 10.1261/rna.7145105. Epub 2004 Dec 1.
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Crystal structure of a group I intron splicing intermediate.I 类内含子剪接中间体的晶体结构
RNA. 2004 Dec;10(12):1867-87. doi: 10.1261/rna.7140504.
7
Structure of the Tetrahymena ribozyme: base triple sandwich and metal ion at the active site.嗜热四膜虫核酶的结构：活性位点处的碱基三联体夹心结构和金属离子
Mol Cell. 2004 Nov 5;16(3):351-62. doi: 10.1016/j.molcel.2004.10.003.
8
Strategies for RNA folding and assembly.RNA折叠与组装策略。
Nat Rev Mol Cell Biol. 2004 Nov;5(11):908-19. doi: 10.1038/nrm1497.
9
Principles of RNA compaction: insights from the equilibrium folding pathway of the P4-P6 RNA domain in monovalent cations.RNA压缩原理：来自单价阳离子中P4-P6 RNA结构域平衡折叠途径的见解
J Mol Biol. 2004 Nov 5;343(5):1195-206. doi: 10.1016/j.jmb.2004.08.080.
10
Monovalent ion-mediated folding of the Tetrahymena thermophila ribozyme.嗜热四膜虫核酶的单价离子介导折叠
J Mol Biol. 2004 Oct 1;342(5):1431-42. doi: 10.1016/j.jmb.2004.07.092.

单价阳离子利用多种机制解决核酶错误折叠问题。

Monovalent cations use multiple mechanisms to resolve ribozyme misfolding.

作者信息

Jiang Yan-Fei, Xiao Mu, Yin Ping, Zhang Yi

机构信息

State Key Laboratory of Virology and Department of Biotechnology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, PR China.

出版信息

RNA. 2006 Apr;12(4):561-6. doi: 10.1261/rna.2188306. Epub 2006 Feb 22.

DOI:10.1261/rna.2188306
PMID:16497656
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1421094/
Abstract

Recent efforts have been made to unravel the independent roles of monovalent cations in RNA folding, primarily using the Tetrahymena ribozyme as a model. Here we report how monovalent cations impact the folding of the Candida ribozyme. Interestingly, this ribozyme requires an order of magnitude less monovalent cations (Na+ and Tris+) to commit to a new folding starting state in which the J3/4:P6 base triple is partially formed and mispairing in the L2.1 and L6 terminal loops is resolved. When Mg2+-induced ribozyme folding proceeded on the same energy landscape, the altered starting state led to a rapid assembly of the correct ribozyme core and a fivefold to 10-fold increase in the ribozyme activity. Moreover, when the ribozyme folding was started from a misfolding-prone state, high millimolar concentrations of monovalent cations moderately elevated the ribozyme activity by efficiently resolving the misfolding of a peripheral element, P5abc.

摘要

最近人们主要利用四膜虫核酶作为模型，努力去揭示单价阳离子在RNA折叠中的独立作用。在此我们报告单价阳离子如何影响念珠菌核酶的折叠。有趣的是，这种核酶在进入一种新的折叠起始状态时所需的单价阳离子（Na⁺和Tris⁺）数量级要少一个，在这种状态下J3/4:P6碱基三联体部分形成，L2.1和L6末端环中的错配得以解决。当Mg²⁺诱导的核酶折叠在相同的能量态势上进行时，改变后的起始状态导致正确的核酶核心快速组装，核酶活性提高了五到十倍。此外，当核酶折叠从易于错误折叠的状态开始时，高毫摩尔浓度的单价阳离子通过有效解决外围元件P5abc的错误折叠，适度提高了核酶活性。