尼古丁治疗可改变人巨细胞病毒感染的ARPE-19细胞中NF-κB的表达。
Nicotine treatment alters NF-kappaB expression in human cytomegalovirus-infected ARPE-19 cells.
作者信息
Buckner Anissa E, Dix Richard D
机构信息
Department of Ophthalmology, Harvey & Bernice Jones Eye Institute, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA.
出版信息
Curr Eye Res. 2006 Feb;31(2):191-8. doi: 10.1080/02713680500514651.
PURPOSE
To test if nicotine counteracts the dampening effect of human cytomegalovirus (HCMV) infection of NF-kappaB in retinal pigment epithelial (RPE) cells, thereby increasing the permissiveness of RPE cells for HCMV replication.
METHODS
Human ARPE-19 cells were transfected with NF-kappaB luciferase DNA, inoculated with HCMV at 24 hr post-transfection, and maintained in the absence or presence of a physiologic dose of nicotine at 1 hr prior to HCMV inoculation.
RESULTS
Whereas HCMV-infected ARPE-19 cells without nicotine treatment showed a dramatic decrease in NF-kappaB levels, nicotine treatment reduced this decrease but did not abolish it completely. Nicotine treatment of uninfected ARPE-19 cells had no effect on baseline NF-kappaB levels.
CONCLUSIONS
Treatment of HCMV-infected ARPE-19 cells with nicotine at a physiologic dose dampened the downregulation of NF-kappaB observed in HCMV-infected ARPE-19 cells without nicotine treatment. We conclude that nicotine can serve as a cofactor to stimulate productive, lytic replication of HCMV.
目的
检测尼古丁是否能抵消人巨细胞病毒（HCMV）感染对视网膜色素上皮（RPE）细胞中核因子κB（NF-κB）的抑制作用，从而增加RPE细胞对HCMV复制的易感性。
方法
将NF-κB荧光素酶DNA转染到人ARPE-19细胞中，在转染后24小时接种HCMV，并在接种HCMV前1小时在有无生理剂量尼古丁的情况下进行培养。
结果
未用尼古丁处理的HCMV感染的ARPE-19细胞中NF-κB水平显著下降，而尼古丁处理减少了这种下降，但并未完全消除。尼古丁处理未感染的ARPE-19细胞对基线NF-κB水平无影响。
结论
用生理剂量的尼古丁处理HCMV感染的ARPE-19细胞，可减轻在未用尼古丁处理的HCMV感染的ARPE-19细胞中观察到的NF-κB的下调。我们得出结论，尼古丁可作为一种辅助因子来刺激HCMV的有效、裂解性复制。
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