利用经典猪瘟病毒候选表位疫苗探寻E2 N端的中和表位。
Spying the neutralizing epitopes on E2 N-terminal by candidate epitope-vaccines against classical swine fever virus.
作者信息
Dong Xiao-Nan, Chen Ying-Hua
机构信息
Laboratory of Immunology, Department of Biology, Tsinghua University, Protein Science Laboratory of the Ministry of Education, Beijing 100084, PR China.
出版信息
Vaccine. 2006 May 8;24(19):4029-34. doi: 10.1016/j.vaccine.2006.01.003. Epub 2006 Jan 13.
Our previous study proved that the N-terminal (aa693-711) of glycoprotein E2 contained sequential neutralizing epitopes. In this study, four candidate epitope-vaccines (EVs) were separately prepared and evaluated. Among them, epitope-vaccine EV-BC1a (BC1a: aa693-699) induced high level of epitope-specific neutralizing antibodies and exhibited similar protective capability with that induced by Chinese vaccine strain (C-strain). These results confirmed CKEDYRY (aa693-699) as a principal sequential neutralizing epitope on E2 N-terminal. Moreover, these findings also indicate that epitope-vaccine is a potent candidate strategy for marker vaccine against classical swine fever virus (CSFV).
我们之前的研究证明，糖蛋白E2的N端（氨基酸693 - 711）含有连续的中和表位。在本研究中，分别制备并评估了四种候选表位疫苗（EVs）。其中，表位疫苗EV - BC1a（BC1a：氨基酸693 - 699）诱导产生了高水平的表位特异性中和抗体，并且表现出与中国疫苗株（C株）诱导的抗体相似的保护能力。这些结果证实CKEDYRY（氨基酸693 - 699）是E2 N端主要的连续中和表位。此外，这些发现还表明，表位疫苗是针对经典猪瘟病毒（CSFV）的标记疫苗的有力候选策略。
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