Slit在果蝇心脏形态发生过程中发挥作用。

Slit coordinates cardiac morphogenesis in Drosophila.

作者信息

MacMullin Allison, Jacobs J Roger

机构信息

Department of Biology, McMaster University, LSB 429, 1280 Main St. W., Hamilton, ON, Canada L8S 4K1.

出版信息

Dev Biol. 2006 May 1;293(1):154-64. doi: 10.1016/j.ydbio.2006.01.027. Epub 2006 Mar 3.

DOI:10.1016/j.ydbio.2006.01.027

PMID:16516189

Abstract

Slit is a secreted guidance cue that conveys repellent or attractive signals from target and guidepost cells. In Drosophila, responsive cells express one or more of three Robo receptors. The cardial cells of the developing heart express both Slit and Robo2. This is the first report of coincident expression of a Robo and its ligand. In slit mutants, cardial cell alignment, polarization and uniform migration are disrupted. The heart phenotype of robo2 mutants is similar, with fewer migration defects. In the guidance of neuronal growth cones in Drosophila, there is a phenotypic interaction between slit and robo heterozygotes, and also with genes required for Robo signaling. In contrast, in the heart, slit has little or no phenotypic interaction with Robo-related genes, including Robo2, Nck2, and Disabled. However, there is a strong phenotypic interaction with Integrin genes and their ligands, including Laminin and Collagen, and intracellular messengers, including Talin and ILK. This indicates that Slit participates in adhesion or adhesion signaling during heart development.

摘要

Slit是一种分泌型导向信号分子，可传递来自靶细胞和路标细胞的排斥或吸引信号。在果蝇中，响应细胞表达三种Robo受体中的一种或多种。发育中心脏的心肌细胞同时表达Slit和Robo2。这是关于Robo及其配体同时表达的首次报道。在Slit突变体中，心肌细胞的排列、极化和均匀迁移受到破坏。Robo2突变体的心脏表型相似，但迁移缺陷较少。在果蝇神经元生长锥的导向过程中，Slit和Robo杂合子之间以及与Robo信号传导所需基因之间存在表型相互作用。相比之下，在心脏中，Slit与包括Robo2、Nck2和Disabled在内的Robo相关基因几乎没有或没有表型相互作用。然而，与整合素基因及其配体（包括层粘连蛋白和胶原蛋白）以及细胞内信使（包括塔林蛋白和整合素连接激酶）存在强烈的表型相互作用。这表明Slit在心脏发育过程中参与黏附或黏附信号传导。