Kurtz A, della Bruna R D
Physiologisches Institut der Universität Zürich, Switzerland.
Kidney Int Suppl. 1991 Jun;32:S13-5.
In this study we examined the effects of classic second messenger molecules on renin secretion and renin synthesis in primary cultures of mouse renal juxtaglomerular (JG) cells. Stimulation of cAMP formation by forskolin, inhibition of calmodulin by calmidazolium, and inhibition of Na+/H+ exchange by ethylisopropylamiloride enhanced renin secretion. Raising of intracellular cGMP by 8-bromo-cGMP and activation of protein kinase C by phorbol ester led to an inhibition of secretion. Renin synthesis was stimulated by forskolin. Calmidazolium, EIPA, 8-bromo-cGMP, and phorbol ester were without effect on basal renin synthesis. The data suggest that renin secretion is influenced by a number of transmembrane transduction systems which in their majority exert a negative control on renin secretion. Activation of adenylate cyclase appears to be a stimulatory control mechanism for both the secretion and the synthesis of renin. The findings suggest, moreover, that the second messenger controls of renin secretion and renin synthesis are not strictly linked in renal JG cells.
在本研究中，我们检测了经典第二信使分子对原代培养的小鼠肾球旁（JG）细胞中肾素分泌和肾素合成的影响。福斯可林刺激cAMP生成、氯丙咪嗪抑制钙调蛋白以及乙基异丙基氨氯吡咪抑制Na+/H+交换均可增强肾素分泌。8-溴-cGMP提高细胞内cGMP水平以及佛波酯激活蛋白激酶C均导致分泌受到抑制。福斯可林刺激肾素合成。氯丙咪嗪、乙基异丙基氨氯吡咪、8-溴-cGMP和佛波酯对基础肾素合成无影响。数据表明，肾素分泌受多种跨膜转导系统影响，其中大多数对肾素分泌发挥负性控制作用。腺苷酸环化酶的激活似乎是肾素分泌和合成的一种刺激控制机制。此外，这些发现表明，肾球旁细胞中肾素分泌和肾素合成的第二信使控制并非严格相关。
