测定游离谷氨酸含量估算花生仁中的真菌污染。
Estimation of fungal infection of peanut kernels by determination of free glutamic Acid content.
出版信息
Appl Environ Microbiol. 1997 Mar;63(3):1083-7. doi: 10.1128/aem.63.3.1083-1087.1997.
Peanut kernels (Tainan 9, a Spanish cultivar) inoculated with Aspergillus parasiticus, A. flavus, A. niger, or A. ochraceus as well as noninoculated kernels were incubated in a humidified environment (relative humidity, 100%) at 25(deg)C for 7 weeks. Internal fungal populations and changes in moisture and sucrose content and free amino acid composition of the kernels were determined periodically. Fungal populations determined by using A. flavus and A. parasiticus agar and rose bengal chlortetracycline agar as enumerating media were closely correlated. Moisture content in the kernels increased from 5.8 to 20.4% (dry basis), and changes in individual free amino acid contents varied, depending upon the incubation time and type of fungus used as an inoculum. In the early infection period (up to 5 weeks), sucrose contents and logarithms of threonine and tyrosine contents increased while logarithms of free glutamic acid content decreased linearly with incubation time. A negative linear relationship was further obtained between logarithms of fungal populations and the logarithm of free glutamic acid content (R(sup2) > 0.80) of the infected peanut kernels.
花生仁（台南 9 号，西班牙品种）接种黄曲霉、寄生曲霉、黑曲霉或杂色曲霉以及未接种的花生仁在相对湿度为 100%的潮湿环境中于 25°C 下孵育 7 周。定期测定内部真菌种群以及水分、蔗糖含量和仁游离氨基酸组成的变化。使用黄曲霉和寄生曲霉琼脂和孟加拉玫瑰红氯四环素琼脂作为计数培养基测定的真菌种群密切相关。仁中的水分含量从 5.8%增加到 20.4%（干基），个别游离氨基酸含量的变化取决于孵育时间和用作接种物的真菌类型。在早期感染期（长达 5 周），蔗糖含量和苏氨酸和酪氨酸含量的对数线性增加，而游离谷氨酸含量的对数线性减少。进一步获得了感染花生仁的真菌种群对数与游离谷氨酸含量对数之间的负线性关系（R(sup2) > 0.80）。
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